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从新生儿脐血和成人骨髓中分选出造血干／祖细胞（ＨＳＣ／ＨＰＣ），构建成ｃＤＮＡ文库，对其进行大规模表达序列标签（ＥＳＴ）测序，通过生物信息学等手段分析基因表达谱，并进行新基因的全长ｃＤＮＡ克隆。在所测的１０５１２条可分析ＥＳＴ序列中，有９８６６条来自脐血ＣＤ３４＋细胞，其中４６９７条（４７６％）为已知基因，２６０３条（２６４％）为已知ＥＳＴ，１４１５条（１４３％）代表未知ＥＳＴ。在已知基因中，８２％基因与造血相关，２２７％涉及细胞代谢、结构和迁移，１３０％与细胞分裂和防御相关，２６２％与ＲＮＡ、蛋白质的合成相关，１０６％和细胞信号传递有关。对一些已知和未知的ＥＳＴ，综合测序、生物信息学等方法，进行全长克隆，已获得２３个新基因的全长ｃＤＮＡ。
Hematopoietic stem / progenitor cells (HSC / HPC) were sorted from neonatal cord blood and adult bone marrow to construct a cDNA library. Large-scale expressed sequence tags (ESTs) were sequenced and analyzed by bioinformatics methods Spectrum, and full-length cDNA cloning of the new gene. Of 105105 ESTs tested, 9866 were from cord blood CD34 + cells, of which 4697 (47.6%) were known, 2603 (26.4%) were ESTs and 1415 (143%) represents unknown EST. Of the known genes, 8.2% were related to hematopoiesis, 22.7% were related to cell metabolism, structure and migration, 13.0% were related to cell division and defense, 26.2% were related to RNA and protein synthesis, 10.6% and cell signaling related. Full-length cloning of some known and unknown ESTs, integrated sequencing, bioinformatics and other methods has resulted in the full-length cDNA of 23 new genes.