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目的: 观察慢性膀胱出口梗阻后P2X1 受体的表达水平.方法: 雌性Wistar大鼠70只,体质量250~300g, 40只为实验组, 30只同龄雌性Wistar大鼠为对照组.在部分膀胱出口梗阻的动物模型成功建立后8wk开始试验,采用免疫组织化学方法结合IMAGE PRO图像处理软件对大鼠膀胱P2X1的表达(即阳性颗粒在某个视野下所占的百分比)进行定量研究.结果: 部分膀胱出口梗阻后大鼠较对照组大鼠膀胱的体积增大,黏膜层增厚,P2X1 受体的表达水平平均为在膀胱平滑肌层[ (9 .60±4 .78)vs(2. 31±1 .19) ] (P<0. 01),在膀胱黏膜层[ (0. 12±0. 03)vs(0 .06±0 .02) ] (P<0 .01),浆膜层[ (0 .04±0. 02 ) vs( 0. 03±0 .01 ) ] (P> 0 .05 ),尿道[ (6 .05±2 .25)vs(1 .48±0 .14) ] (P<0. 01),慢性膀胱出口梗阻后P2X1 受体的表达水平均明显高于对照组.结论: 膀胱出口部分梗阻后,P2X1 受体在膀胱各个部位表达均升高.推测P2X1 可能参与了慢性膀胱出口梗阻后膀胱的生理或病理功能的调节.
Objective: To observe the expression of P2X1 receptor after chronic bladder outlet obstruction.Methods: Seventy female Wistar rats weighing 250-300g were used as the experimental group and 30 female Wistar rats of the same age as the control group, The animal model of outlet obstruction was successfully established 8 weeks after the start of the experiment, the immunohistochemical method combined with IMAGE PRO image processing software on rat bladder P2X1 expression (ie positive particles in a field of view) as a quantitative study.Results: Part of the bladder outlet obstruction in rats than the control group rats bladder volume increased mucosal thickening, P2X1 receptor expression levels in the bladder smooth muscle layer [(9 .60 ± 4.78) vs (2.31 (P <0.01), but not in the bladder mucosa [(0.12 ± 0.03) vs (0.06 ± 0.02)] (P <0.01) (P> 0.05), urethra (6 .05 ± 2 .25) vs (1.48 ± 0.14) (P <0.01), the expression level of P2X1 receptor in chronic bladder outlet obstruction was significantly higher than that in control group.Conclusion: The expression of P2X1 receptor in all parts of bladder increased after partial obstruction of bladder outlet.It is speculated that P2X1 may Participated in chronic bladder outlet stems Adjusting the physiological or pathological function after bladder.