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用ＷＱ－９Ｅ钾离子透入仪筛选出对痛刺激敏感的ＳＤ大鼠８只，随机分成对照组及针刺组，每组４只。针刺鼠用ＷＱ－９Ｅ电针仪刺激两侧＂足三里＂穴，持续３０ｍｉｎ。所有动物同时用１％戊巴比妥钠腹腔内麻醉后开胸，用Ｂｏｕｉｎ液经心灌注固定，开颅取脑，作连续冰冻切片（片厚４０μｍ），隔片取一。所有切片均按Ｓｔｅｒｎｂｅｒｇｅｒ（ＰＡＰ）法进行免疫细胞化学反应，ＤＡＢ呈色，封片后直接连ＩＢＭ２８６－ＣＡＰ５３０图像分析系统对视上核，室旁核催产素免疫反应阳性物质作定量测定。结果显示：针刺鼠下丘脑视上核，室旁核催产素免疫反应阳性物质的总面积比对照鼠分别增加了４４．１％、４７．３％（Ｐ＜０．０１）。提示，催产素免疫反应阳性物质的增加与电针刺激足三里穴有关，催产素可能参与电针镇痛的中枢调节过程。
Eight SD rats sensitive to pain stimulation were screened by WQ-9E potassium iontophoresis instrument, and randomly divided into control group and acupuncture group, 4 rats in each group. Acupuncture rats with WQ-9E electroacupuncture instrument to stimulate the “Zusanli” points on both sides, for 30min. All animals were simultaneously anesthetized with 1% sodium pentobarbital and then thoracotomy. The animals were anesthetized by intraperitoneal injection of 1% sodium pentobarbital. The animals were perfused with Bouin fluid, and craniotomy was used to make continuous frozen sections (thickness: 40 μm). Immunocytochemistry was performed on all sections according to the Sternberger (PAP) method. DAB was colorized and directly labeled with the IBM 286-CAP530 image analysis system for the quantitative determination of supraoptic nucleus and paraventricular nucleus oxytocin-immunoreactive substances. The results showed that the total area of supraoptic nucleus and paraventricular nucleus oxytocin immunoreactive substances in acupuncture rats increased by 44.1% and 47.3% respectively (P <0.01) compared with control rats. Tip, the increase in oxytocin-immunoreactive substances and electroacupuncture at Zusanli point, oxytocin may be involved in the central regulation of electroacupuncture analgesia.