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目的 探讨纤维支气管镜标本的p5 3基因突变检测对老年肺癌早期诊断的价值。方法 应用聚合酶链反应—单链构象多肽性分析 (PCR—SSCP)技术 ,对 5 4例临床高度疑诊肺癌的老年患者纤维支气管镜标本进行p5 3基因 5～ 8号外显子突变分析 ,分析结果与病理学对照或随访以证实阳性的可信度。结果 19例确诊老年肺癌患者中 ,有 10例 p5 3基因发生突变 ,占 5 3%。在 35例疑诊老年肺癌但病理学阴性的患者中发生p5 3基因突变的 11例 ,无p5 3基因突变的 2 4例。经过 2 8个月的随访 ,p5 3基因突变组有 5例证实为肺癌 ,占 4 6 % ;而无p5 3基因突变组只有 2例证实为肺癌 ,占 9%。两组比较 ,差异具有显著性 (P <0 0 1)。结论 通过对患者纤维支气管镜标本的 p5 3基因突变分析 ,作为早期诊断老年肺癌的一种方法是可行的
Objective To investigate the value of p5 3 gene mutation detection in early diagnosis of lung cancer in patients with bronchoscopy. Methods PCR-SSCP was used to analyze the mutation of p5 3 gene exons 5-8 in 54 cases of elderly patients with highly suspected lung cancer with PCR-SSCP. Results and pathology control or follow-up to confirm the credibility of the positive. Results Of the 19 cases of elderly patients diagnosed with lung cancer, 10 cases of p5 3 gene mutation, accounting for 53%. Eleven patients with p5 3 gene mutation and 24 patients without p5 3 gene mutation were found in 35 patients with suspected lung cancer but pathologically negative. After 2 months and 8 months of follow-up, 5 cases of p5 3 gene mutation group confirmed lung cancer, accounting for 46%. Only 2 cases without p5 3 gene mutation group confirmed lung cancer, accounting for 9%. The difference between the two groups was significant (P <0.01). Conclusion It is feasible to diagnose senile lung cancer by analyzing the mutation of p5 3 gene in patients with bronchoscopy