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目的对安康市2012-2014年流行期流感病毒进行分离鉴定及分析,了解安康市流感流行动态,探索流行规律。方法采集流感样病例(ILI)的咽拭子标本先用real-time PCR检测,阳性标本用狗肾传代细胞(MDCK)进行病毒分离,采用血凝抑制方法(HI)进行流感病毒型别鉴定。结果 2012年10月至2014年3月共监测1所哨点医院ILI咽拭子标本905份,其中PCR阳性171份;分离出流感病毒97株,其中新甲型H1N1 62株,季节性A(H3N2)11株,B型(yamagata)24株。结论 2012-2014年流感流行季节中安康市流行的优势毒株为新甲型H1N1,12月至次年1月为流行高峰,有其他型别的交替流行。
OBJECTIVE To isolate and identify influenza viruses in the epidemic period of 2012-2014 in Ankang City and to understand the epidemic trend of influenza in Ankang City and to explore the epidemic law. Methods Throat swab samples of influenza-like illness (ILI) were collected and detected by real-time PCR. Positive samples were isolated by MDCK and identified by HI. Results A total of 905 ILI throat swabs from 1 sentinel hospital were monitored from October 2012 to March 2014, of which 171 were positive for PCR. 97 strains of influenza virus were isolated, of which 62 were new type A (H1N1), seasonal A (A 11 strains of H3N2 and 24 strains of B type (yamagata). Conclusions The predominant strain prevailing in Ankang City during 2012-2014 was Influenza A H1N1. The epidemic peak was in January and January January, and there were other types of epidemic strains.