目的:检测JNK1在小鼠植入期子宫中的表达,探讨JNK1的表达与胚胎植入的关系。方法:30只昆明雌鼠,随机分为孕0.5天、4.5天、6.5天组并合笼,取得子宫标本,应用Realtime-PCR方法测定小鼠植入期子宫中JNK1基因的表达,应用链霉菌抗生物素蛋白-过氧化物酶连接(SP)法定位检测JNK1蛋白在小鼠植入期子宫中的表达。结果:与植入前相比,JNK1在小鼠植入开始时(孕4.5天)表达量增加,是植入前(孕0.5天)的1.5倍,至植入完毕后表达量减少,是植入完毕(孕6.5天)后的2倍;并且JNK1在子宫内膜中是阳性表达的。结论:JNK1在小鼠植入期子宫中表达量增加,提示JNK1在胚胎植入过程中可能有重要作用。 OBJECTIVE: To detect the expression of JNK1 in the uterus of mice during implantation and to explore the relationship between JNK1 expression and embryo implantation. Methods: Thirty female Kunming mice were randomly divided into pregnant 0.5, 4.5 and 6.5 days group and were caged to obtain the uterus specimens. The expression of JNK1 gene in the uterus during implantation was detected by Realtime-PCR. Avidin-peroxidase linked (SP) method was used to detect the expression of JNK1 protein in the uterus during implantation. Results: Compared with the pre-implantation, the expression of JNK1 increased 1.5-fold before implantation (4.5 days pregnant) and decreased after the implantation 2 fold after completion (6.5 days pregnant); and JNK1 is positively expressed in the endometrium. Conclusion: The expression of JNK1 in the uterus of mice during implantation increased, suggesting that JNK1 may play an important role in embryo implantation.