GABA能与PV能神经元在中枢神经内的分布有很高的一致性,且PV在小脑的含量又为其它部位的10倍以上,但人小脑内GABA能神经元与PV能神经元是否也有共存,以及它们彼此配布的功能意义,目前尚有待研究阐明。本实验用免疫细胞化学ABC法,结合Bordin’s镀银复染,研究人胎小脑GABA和PV能神经元及其纤的发育.结果所见16周龄,GABA阳性神经元不仅见于小脑皮质的星形细胞,兰细胞、浦肯野细胞和颗粒细胞,而且还见于脑小叶内白质有棱形的阳性神经元.16周龄,PV反应阳性则仅见散在于浦肯野细胞有隐约存在.23周龄后,GABA阳性的浦肯野细胞已十分明显,且体积增长,突起延长并出现纤维分叉等形态学的成熟过程。 GABA and PV can be distributed in the central nervous system neurons have a high degree of consistency, and the content of PV in the cerebellum and other parts of more than 10 times, but the human cerebellar GABA neurons and PV neurons also have Coexistence, and the function of their distribution with each other, it is yet to be elucidated. In this study, immunocytochemical ABC method combined with Bordin’s silver staining to study human fetal cerebellum GABA and PV neurons and their fiber development.Results 16 weeks of age, GABA positive neurons not only seen in the cerebellar cortex of the star Cells, Lan cells, Purkinje cells and granulosa cells, but also found in the lobular white matter with prismatic positive neurons.With 16 weeks of age, PV positive reaction only scattered scattered in Purkinje cells looming.23 weeks old After GABA-positive Purkinje cells have been very clear, and the volume growth, protuberance and fiber maturity morphological maturation occurs.