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目的:建立高效液相色谱法测定黄栀花口服液中栀子苷。方法:采用Kromasil C18色谱柱,流动相为甲醇-水(62∶38),检测波长为238nm,流速1.0mL·min-1,柱温为30℃。结果:栀子苷在5.44～162.84μg·mL-1范围内线性关系良好,r=0.9997;平均回收率为98.4%,RSD为0.75%(n=6)。结论:方法简便、准确、重复性好,适用于黄栀花口服液的质量控制。
Objective: To establish a HPLC method for determination of geniposide in huangzhihua oral liquid. Methods: Kromasil C18 column was used. The mobile phase was methanol-water (62:38). The detection wavelength was 238 nm and the flow rate was 1.0 mL · min-1. The column temperature was 30 ℃. Results: Geniposide had a good linearity in the range of 5.44-162.84 μg · mL-1, r = 0.9997; the average recovery was 98.4% with a RSD of 0.75% (n = 6). Conclusion: The method is simple, accurate and reproducible. It is suitable for the quality control of.