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为明确人乳头瘤病毒(HPV)与人宫颈癌的关系及致癌的分子机理,取自宫颈癌高发区新疆、华北;中发区湖北、黑龙江;低发区贵州的宫颈癌318例,慢性宫颈炎39例,湿疣86例,宫颈上皮内新生物(CIN)14例及正常宫颈上皮24例在用斑点、Southern blot。原位核酸杂交进行HPV—11,16及18基因检测的同时进行同一标本的电镜对比观察。为更精确检测HPV基因及探素早期基因诊断的新法又发展了改良PCR对106例宫颈癌(新疆73,华北33例),湿疣72例
In order to clarify the relationship between human papillomavirus (HPV) and human cervical cancer and the molecular mechanism of carcinogenesis, we choose from the high incidence areas of cervical cancer in Xinjiang and North China; 318 cases of cervical cancer in Guizhou and low incidence areas of Hubei and Heilongjiang; 39 cases of inflammation, 86 cases of genital warts, cervical intraepithelial neoplasia (CIN) in 14 cases and normal cervical epithelium in 24 cases with spots, Southern blot. In situ hybridization of nucleic acids for HPV-11, 16 and 18 gene detection at the same time the same specimen electron microscopy comparison. In order to detect HPV genotypes and their early genes more accurately, a modified PCR was developed for 106 cases of cervical cancer (73 in Xinjiang and 33 in North China), and 72 cases of genital warts