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作者观察了犬冠状动脉急性闭塞再灌注过程中中性白细胞膜流动性及血清肌酸磷酸激酶（ＣＰＫ）的变化．结果表明：冠状动脉左前降支（ＬＡＤ）阻断后，心大静脉回流血中中性白细胞膜荧光偏振Ｐ值升高，阻断前为０．２７±０．０１，阻断１５ｍｉｎ为０．２９±０．０１，中性白细胞膜流动性下降．随缺血时间延长，中性白细胞膜荧光偏振Ｐ值明显升高，阻断３０ｍｉｎ为０．３０±０．０１，阻断６０ｍｉｎ为０．３２±０．０１（与阻断１５ｍｉｎ比Ｐ＜０．０５）．再灌注后，中性白细胞膜荧光偏振Ｐ值继续升高．中性白细胞荧光偏振Ｐ值与血清ＣＰＫ浓度呈正相关（ｒ＝０．８９，Ｐ＜０．０５）．结论：心肌缺血再灌注过程中中性白细胞膜流动性降低，并与血清ＣＰＫ值密切相关．
The authors observed changes in neutrophil membrane fluidity and serum creatine phosphokinase (CPK) during acute coronary occlusion and reperfusion in dogs. The results showed that after the left anterior descending coronary artery (LAD) was occluded, the fluorescence polarization P value of neutrophil membrane in the blood returning from the cardiac vena cava increased, with a mean of 0.27 ± 0.01 before the blockade and 0 after the blockade within 15 minutes. 29 ± 0.01, neutrophil membrane fluidity decreased. With the prolongation of ischemia time, the P value of fluorescence intensity of neutrophil membrane was significantly increased, with a mean of 0.30 ± 0.01 for 30min, 0.32 ± 0.01 for 60min (P <0 .05). After reperfusion, the fluorescence polarization P value of neutrophil membrane continued to increase. P value of neutrophil fluorescence polarization was positively correlated with serum CPK concentration (r = 0.89, P <0.05). Conclusion: Neutrophil membrane fluidity decreases during myocardial ischemia-reperfusion and is closely related to serum CPK value.