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采用傅里叶变换红外光谱(FTIR)、二阶导数谱结合二维相关红外光谱(2D-IR),对8种豆样品(黄豆、黑豆、蚕豆、豌豆、白芸豆、红豆、绿豆和红小豆)进行鉴别分析。结果显示,8种豆的原始光谱吸收峰非常相似,仅在吸收峰强度和峰位上有微小差异。对1700~800 cm-1范围的光谱进行二阶导数处理,8种豆的二阶导数谱差异明显。对8种豆样品进行2D-IR光谱分析,发现在860~1250 cm-1和1400~1700 cm-1波段内,8种豆在自动峰的位置、数量和强度上均存在明显差异。结果表明,应用红外光谱、二阶导数谱和二维相关红外光谱技术可以快速有效地分析和鉴别不同种类的豆。因此红外光谱分析方法对于豆类的分类鉴别是一种快速、准确、有效的方法。
Eight soybean samples (soybean, black bean, broad bean, pea, white kidney bean, red bean, mung bean and red adzuki bean ) For differential analysis. The results showed that the original spectra of 8 kinds of beans have very similar absorption peaks, with only slight difference in peak intensity and peak position. The second-order derivative of the spectrum in the range of 1700-800 cm-1 was observed. The differences of the second derivative spectra of the eight kinds of beans were obvious. The results of 2D-IR spectra of 8 kinds of soybean samples showed that there were obvious differences in the position, quantity and intensity of 8 kinds of beans in 860 ~ 1250 cm-1 and 1400 ~ 1700 cm-1 bands. The results show that the application of infrared spectroscopy, second derivative spectra and two-dimensional correlation infrared spectroscopy can quickly and effectively analyze and identify different types of beans. Therefore, the infrared spectroscopy method for the classification of legumes is a fast, accurate and effective method.