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目的:对第4代航天诱变育种桔梗固有成分及微结构进行测定表征,以获得航天诱变对其固有成分及微结构影响的信息,为筛选优质药用植物新品种提供依据。方法:联用X射线荧光(XRF)、粉末X射线衍射(PXRD)和傅里叶变换红外光谱分析(FTIR)技术对太空组和地面组桔梗进行对比分析,从多种元素种类及含量、原子和分子层面表征其固有成分。结果:太空桔梗中Mn、Zn、Fe、Ca、Mg元素含量比地面组明显提高,Al元素含量降低;PXRD和FTIR谱的特征有一定的差异,可区别太空组桔梗与地面组桔梗固有成分的变化。太空桔梗已不含菊糖,而非晶体物质的主要活性基团组分有所增加。结论:航天诱变育种有利于选育出变异幅度大的特异质的桔梗新品种。3种技术联用可用于药材优良品种的选育及固有成分稳定性的控制。
OBJECTIVE: To obtain the information of the inherent composition and microstructure of spaceflight mutagenesis in the fourth generation of space-mutation breeding of Platycodon regia, and to provide the basis for screening new medicinal plants of high quality. Methods: The relatives of space group and ground group were analyzed by XRF, PXRD and FTIR. The results showed that the content of various elements, And molecular characterization of its inherent components. Results: The contents of Mn, Zn, Fe, Ca and Mg in space crown of Platycodon grandiflorum were significantly higher than those of the ground group and the content of Al was decreased. The characteristics of PXRD and FTIR spectrum were different, Variety. Campanulaceae is free of inulin and the major active group components of non-crystalline materials increase. Conclusion: Space mutation breeding is conducive to the breeding of a large variety of special mutant Campanulaceae new varieties. Three kinds of technology can be used for breeding superior varieties of medicinal herbs and the stability of the inherent stability control.