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我们用日本第一化学公司的 BA 试剂盒,在 COBAS-FARA-Ⅱ全自动生化分析仪上对原程序作了部分修改,建立了成本低,适用于大批量检测 BA 的全自动方法。主要参数:样品量7μl。试剂量140μl,起动试剂35μl,波长540nm,反应温度30℃。用该法对155例各类病毒性肝炎患者血清作胆酸(Bile acid,BA)检测,结果,对照组(n=43)为5.69±3.7μmol/L;CPH(n=16)为7.63±3.26μmol/L;急性病毒性肝炎(n=39)为:急性期107.78±70.64
We used the first chemical company of Japan BA kit, COBAS-FARA-Ⅱ automatic biochemical analyzer on the part of the original program was modified to establish a low-cost, suitable for high-volume detection of BA fully automatic method. The main parameters: sample volume 7μl. Reagent amount 140μl, starter reagent 35μl, wavelength 540nm, the reaction temperature 30 ℃. The serum of 155 patients with various types of viral hepatitis was detected by Bile acid (BA) method. The results showed that the control group (n = 43) was 5.69 ± 3.7μmol / L and the CPH (n = 16) was 7.63 ± 3.26μmol / L; acute viral hepatitis (n = 39) were: acute stage 107.78 ± 70.64