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目的 研究原发性肝癌患者红细胞免疫粘附肿瘤细胞能力与红细胞ＣＲ１ 基因组密度多态性及活性的相关性。方法 采用ＰＣＲ 和Ｈｉｎｄ Ⅲ酶切技术测定红细胞ＣＲ１ 基因组密度多态性变化，同时用ＥＬＩＳＡ 和肿瘤红细胞花环试验测定红细胞ＣＲ１ 活性，并进行比较。结果 原发性肝癌患者红细胞ＣＲ１ 基因型点突变比率（４３－６ ％ ） 明显上升，与正常人（２０ ％ ） 相比，差异有显著性（ Ｐ＜０－０５） ；原发性肝癌患者３ 种ＣＲ１ 基因型的红细胞ＣＲ１ 活性都明显低于正常人（ Ｐ＜ ０－０１ 或 Ｐ＜０－０５） 。活性变化也不同。结论 原发性肝癌患者红细胞ＣＲ１ 活性及免疫粘附肿瘤细胞能力下降可分为三种不同类型，即原发型、获得型和混合型。
Objective To investigate the correlation between the ability of RBCs to adhere to tumor cells and the polymorphism and activity of CR1 gene density in erythrocytes of patients with primary liver cancer. Methods PCR and Hind III digestion techniques were used to determine the erythrocyte CR1 genome density polymorphisms. Erythrocyte CR1 activity was measured by ELISA and red cell rosette assay, and compared. Results The percentage of CR1 genotype point mutations (43-6 %) in patients with primary liver cancer was significantly higher than that in normal controls (20 %) (P<0-05); Primary liver cancer patients 3 CR1 genotype CR1 activity of red blood cells were significantly lower than normal (P <0-01 or P <0-05). The activity varies. Conclusion The decrease of CR1 activity in red blood cells and the ability of immunoadhesive tumor cells in patients with primary liver cancer can be divided into three different types, ie, primary, acquired and mixed.