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目的:测定明胶和吸收性明胶海绵的总蛋白质含量,为控制和提高产品质量提供依据。方法:分别用双缩脲法、联喹啉二羧酸法和考马斯亮蓝法测定了明胶和吸收性明胶海绵的总蛋白质含量。结果:考马斯亮蓝G250与明胶溶液的显色反应不够灵敏,而与吸收性明胶海绵的水解液不显色。双缩脲法和2,2′-联喹啉-4,4′二羧酸法的测定结果分别为70%～78%和51%～64%。采用上述同一种方法分别测定明胶和吸收性明胶海绵的总蛋白质含量,结果无显著性差异。结论:考马斯亮蓝法不适用于明胶和吸收性明胶海绵的总蛋白质含量测定,而双缩脲法和2,2′-联喹啉-4,4′二羧酸法的测定结果具有参考价值。
Objective: To determine the total protein content of gelatin and absorbent gelatin sponge, to provide the basis for controlling and improving product quality. Methods: The total protein content of gelatin and absorbent gelatin sponge was determined by the biuret method, the biquinoline dicarboxylic acid method and the Coomassie Brilliant Blue method respectively. Results: The color reaction of Coomassie Brilliant Blue G250 with gelatin solution was not sensitive enough, but not with the hydrolyzate of absorbent gelatin sponge. The results of the biuret method and 2,2’-biquinoline-4,4’-dicarboxylic acid method were 70% -78% and 51% -64%, respectively. The same method as above was used to determine the total protein content of gelatin and absorbent gelatin sponge, respectively, with no significant difference. CONCLUSION: Coomassie brilliant blue method is not suitable for the determination of total protein content of gelatin and absorbent gelatin sponge, while the biuret method and 2,2’-biquinoline-4,4 ’dicarboxylic acid method have the reference value .