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目的制备老年期痴呆大鼠模型,并将其应用于老年期痴呆发病机制及其药物疗效方面的研究。方法选取雄性Wistar大鼠52只,将其随机分成5组,分别为双侧颈总动脉永久结扎(2VO)+腹腔注射半乳糖模型组(共有2组,其处理顺序相反)、双侧颈总动脉永久结扎组、腹腔注射半乳糖组及正常对照组;采用Morris水迷宫行为学测试法比较各组大鼠学习和记忆功能间的差异。结果与正常对照组及颈总动脉结扎组大鼠比较,2VO后+腹腔注射半乳糖模型组及半乳糖腹腔注射后+2VO模型组大鼠均出现明显的学习、记忆功能减退现象,差异有统计学意义。结论联合采用双侧颈总动脉永久结扎及半乳糖腹腔注射方法制备的老年期痴呆大鼠模型能有效重现老年期痴呆患者的发病特点,可将其进一步应用于血管性痴呆、阿尔茨海默病及混合性痴呆等老年期痴呆疾病的基础研究中。
Objective To prepare a rat model of senile dementia, and apply it in the pathogenesis of senile dementia and its drug efficacy. Methods Fifty-two male Wistar rats were randomly divided into 5 groups: permanent bilateral carotid artery ligation (2VO) and intraperitoneal injection of galactose (2 groups in total, with the opposite order of treatment), bilateral total neck Permanent ligation group, intraperitoneal injection of galactose group and normal control group; Morris water maze behavioral test was used to compare the learning and memory function of each group. Results Compared with the normal control group and the common carotid artery ligation group, the rats in 2VO plus intraperitoneal injection of galactose and galactose + 2VO model group showed obvious learning and memory loss, the difference was statistically significant Significance of learning. Conclusion The model of senile dementia rats combined with permanent ligation of bilateral common carotid artery and galactose intraperitoneal injection can effectively reproduce the onset characteristics of senile dementia patients and can be further applied to vascular dementia, Alzheimer’s disease Disease and mixed dementia and other senile dementia disease basic research.