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在确定 5种泡桐叶片诱导愈伤组织基本培养基为MS的基础上 ,筛选出了毛泡桐 (Paulowniatomen tosa)、南方泡桐 (Paulowniaaustralis)、白花泡桐 (Paulowniafortunei)、兰考泡桐 (Paulowniaelongata)和豫杂一号泡桐 (P .tomentosa×P .fortunei)叶片愈伤组织诱导的最适培养基 (分别为MS +0 .5NAA +4BA、MS +0 .3NAA +2BA、MS +0 .5NAA +4BA、MS +0 .3NAA +6BA和MS +0 .3NAA +8BA) ,然后 ,从 18个不同浓度NAA和BA组合的MS培养基中 ,找出了它们叶片愈伤组织诱导芽分化最适培养基 (分别为MS +0 .3NAA +12BA、MS +0 .3NAA +12BA、MS +0 .5NAA +12BA、MS +0 .5NAA +12BA和MS +0 .7NAA +12BA) ,最后 ,找出了 5种泡桐芽诱导根的最适培养基 (分别为 1 2MS +0 .1NAA、1 2MS +0 .1NAA、1 2MS、1 2MS +0 .3NAA和 1 2MS +0 .5NAA)。这些结果为开展泡桐基因工程研究和利用不同种泡桐叶片原生质体融合培育泡桐新品种提供了参考
On the basis of determining the basic medium for callus induction of five kinds of Paulownia leaves, we selected Paulowniatomen tosa, Paulownia australis, Paulownia fortunei, Paulownia elongata, (NA) +4 BA, MS +0.3 NAA +2 BA, MS +0.5 NAA +4 BA and MS +0.5 NAA + 4 BA, respectively, were the most suitable medium for the leaf callus induction of P.tomentosa × P. fortunei, 0.3NAA + 6BA and MS +0.3NAA + 8BA). Then, the optimal media for inducing bud differentiation from leaves were obtained from 18 MS medium with different concentrations of NAA and BA (respectively MS +0.3NAA + 12BA, MS +0.3NAA + 12BA, MS +0.5NAA + 12BA, MS +0.5NAA + 12BA and MS +0.7NAA + 12BA). Finally, five kinds of Paulownia buds The optimal medium for induction of roots (1 2 MS + 0.1 NAA, 1 2 MS + 0.1 NAA, 1 2 MS, 1 2 MS + 0.3 NAA and 1 2 MS + 0.5 NAA, respectively). These results provide a reference for the genetic engineering of Paulownia and the use of different varieties of Paulownia leaf protoplast fusion Paulownia new varieties of reference