论文部分内容阅读
目的探讨一氧化氮（ＮＯ）、一氧化氮合酶（ＮＯＳ）、晚期糖化终末产物（ＡＧＥｓ）和超氧化物歧化酶（ＳＯＤ）在衰老中的作用。方法测定２０月龄和３月龄大鼠体内ＮＯ、ＮＯＳ、ＡＧＥｓ和ＳＯＤ的活性。结果２０月龄大鼠骨胶原中ＡＧＥｓ显著增加（Ｐ＜０．０５），血浆中ＮＯ明显高于３月龄对照组，ＮＯＳ活性增高（分别为Ｐ＜０．０２和Ｐ＜０．００１），而ＳＯＤ活性显著降低（Ｐ＜０．０２）。结论从ＮＯ生理功能可知，此结果与老年体内发生的生理变化相矛盾。推测可能由于随着年龄的增长ＳＯＤ合成减少，对Ｏ÷２灭活减少，更多的Ｏ÷２与ＮＯ反应生成ＯＯＮＯ·，使ＮＯ生物学活性降低。另一方面，ＡＧＥｓ通过化学反应直接灭活ＮＯ，故而导致老年体内表现出众多ＮＯ缺乏的体征。
Objective To investigate the role of nitric oxide (NO), nitric oxide synthase (NOS), advanced glycation end products (AGEs) and superoxide dismutase (SOD) in senescence. Methods The activities of NO, NOS, AGEs and SOD in 20 and 3 month old rats were determined. Results The AGEs of 20-month-old rats were significantly increased (P <0.05), and the NO level in plasma was significantly higher than that of 3-month-old controls (P <0.02 and P <0.001, respectively) , While SOD activity decreased significantly (P <0.02). Conclusions From the physiological functions of NO, we can see that the results contradict the physiological changes that occur in the elderly. It is speculated that the reduction of SOD synthesis may be due to the increase of age, the decrease of inactivation of O ÷ 2, and the more O ÷ 2 reacts with NO to form OONO · and the decrease of NO biological activity. On the other hand, AGEs directly inactivate NO through chemical reactions, leading to many signs of NO deficiency in the elderly.