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Objects:The aim of this study was to research the effect of grape seed procyanidin extract(GSPE) on cell apoptosis in human bladder cancer BIU87 cells and investigate its molecular mechanism.Methods:BIU87 cells were treated with different concentrations of GSPE and cultured for 24 h in vitro while untreated group as control,MTT[3-(4,5-dimethylthiazole-2-yl) -2,5-diphenyltetrazolium bromide] assay,Hoechst 33258 stainning,flow cytometry,RT-PCR and Western blot were used to detect the apoptotic induction effect of GSPE on BIU87 cells.Results:We found that GSPE induced cell apoptosis in BIU87 cells by a dose-dependent manner.Semiquantitated RT-PCR and Western blot analyses indicated that GSPE increased the expression of caspase-3 and decreased the expression of survivin(P < 0.01).Conclusion:GSPE induces apoptosis in BIU87 cells in vitro,and the effect maybe related with its down-regulation of survivin.
Objects: The aim of this study was to research the effect of grape seed procyanidin extract (GSPE) on cell apoptosis in human bladder cancer BIU87 cells and investigate its molecular mechanism. Methods: BIU87 cells were treated with different concentrations of GSPE and cultured for 24 h in vitro while untreated group as control, MTT [3- (4,5-dimethylthiazole-2-yl) -2,5-diphenyltetrazolium bromide] assay, Hoechst 33258 stainning, flow cytometry, RT-PCR and Western blot were used to detect the apoptotic induction effect of GSPE on BIU87 cells. Results: We found that GSPE induced cell apoptosis in BIU87 cells by a dose-dependent manner. Semiquantitated RT-PCR and Western blot analyzes indicated that GSPE increased the expression of caspase-3 and decreased the expression of survivin (P <0.01) .Conclusion: GSPE induces apoptosis in BIU87 cells in vitro, and the effect maybe related with its down-regulation of survivin.