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目的：观察白细胞介素－１β（ｉｎｔｅｒｌｅｕｋｉｎ－１β，ＩＬ－１β）和转化生长因子－β（ｔｒａｎｓｆｏｒｍｉｎｇｇｒｏｗｔｈｆａｃｔｏｒ－β，ＴＧＦ－β）单独作用、组合后对人牙周膜成纤维细胞（ｈｕｍａｎｐｅｒｉｏｄｏｎｔａｌｌｉｇａｍｅｎｔｆｉｂｒｏｂｌａｓｔ，ＨＰＬＦ）的ＤＮＡ合成量的影响。方法：用３Ｈ胸腺嘧啶脱氧核苷细胞内掺入法。结果：ＤＮＡ合成量显著增多。浓度为０．０１ｎｇ／ｍｌ的ＴＧＦ－β与浓度为１０ｕ／ｍｌ的ＩＬ－１β组合后对ＨＰＬＦ的ＤＮＡ合成量与单纯ＩＬ－１β组间无显著差异（Ｐ＞０．０５），而０．１ｎｇ／ｍｌ、１．０ｎｇ／ｍｌ的ＴＧＦ－β与三种浓度ＩＬ－１β组合后，ＨＰＬＦ的ＤＮＡ合成量显著高于单纯的ＩＬ－１β组。结论：ＩＬ－１β、ＴＧＦ－β对ＨＰＬＦ的ＤＮＡ合成有促进作用，这两种细胞因子组合并非各个细胞因子作用的简单相加
OBJECTIVE: To observe the effects of interleukin-1β (IL-1β) and transforming growth factor-β (TGF-β) alone on human periodontal fibroblasts (HPLF) Of the amount of DNA synthesis. Methods: 3H thymidine incorporation method. Results: DNA synthesis increased significantly. There was no significant difference (P> 0.05) between the amount of DNA synthesis of HPLF and the concentration of TGF-β of 0.01ng / ml and the concentration of IL-1β of 10u / ml. 1ng / ml, 1.0ng / ml TGF-β combined with three concentrations of IL-1β, HPLF DNA synthesis was significantly higher than the simple IL-1β group. CONCLUSION: IL-1β and TGF-β can promote the DNA synthesis of HPLF. The combination of these two cytokines is not a simple addition of each cytokine