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目的:通过检测三硝基环磺酸(TNBS)诱导的结肠炎大鼠结肠组织中TL1A、NF-κB的表达及5-氨基水杨酸(5-ASA)干预后的表达,探讨它们和克罗恩病(CD)之间的关系及5-ASA干预后的影响。方法:选用雌性健康Wistar大鼠30只,均分为A、B、C组。A为正常对照组,B、C两组大鼠采用TNBS/乙醇灌肠制作大鼠结肠炎模型。造模后,B组每天给予0.9%氯化钠溶液1ml灌肠;C组每天给予5-ASA 1ml灌肠(100mg/kg)。于造模后第7天处死所有大鼠,按疾病活动指数(DAI)的评分标准进行大体损伤评分,HE染色进行组织损伤评分。同时取结肠病变部位组织,生化法检测MPO活性,应用荧光定量PCR检测TL1A、NF-κB的表达的变化。结果:与A组比较,B组和C组的DAI评分、大体损伤形态和组织学损伤评分及MPO活性均升高(P<0.05),但B组高于C组(P<0.05)。与A组相比,B、C组的TL1A、NF-κB水平升高(P<0.05),分别为0.09±0.51比0.91±0.17和0.35±0.05、0.11±0.06比0.82±0.17和0.33±0.14,且B组高于C组。结论:在TNBS诱导的大鼠炎性肠病模型中TL1A、NF-κB的表达增高,5-ASA对肠道炎症的治疗作用可能是通过抑制TL1A、NF-κB实现的。
Objective: To investigate the expression of TL1A and NF-κB in colonic tissues induced by TNBS and the expression of TL-5A and 5-ASA in colonic tissues of rats with colitis induced by TNBS, Ron disease (CD) and the impact of 5-ASA intervention. Methods: Thirty female Wistar rats were randomly divided into A, B and C groups. A is a normal control group, B, C two groups of rats using TNBS / ethanol enema rat colitis model. After modeling, group B was given 1ml of 0.9% sodium chloride solution enema every day; Group C was given 1ml of 5-ASA enema (100mg / kg) every day. All rats were sacrificed on the 7th day after establishment of the model. Gross lesion score was calculated according to the score of disease activity index (DAI), and tissue damage score was made by HE staining. At the same time take the parts of colonic lesions, biochemical detection of MPO activity, the use of quantitative real-time PCR detection TL1A, NF-κB expression changes. Results: Compared with group A, the DAI score, gross lesion score, histological damage score and MPO activity in group B and group C were significantly higher than those in group A (P <0.05), but were significantly higher in group B than those in group C (P <0.05). The levels of TL1A and NF-κB in group B and group C were significantly higher than those in group A (P <0.05), which were respectively 0.09 ± 0.51 and 0.91 ± 0.17 and 0.35 ± 0.05, 0.11 ± 0.06 and 0.82 ± 0.17 and 0.33 ± 0.14 , And group B was higher than group C Conclusion: The expression of TL1A and NF-κB is increased in TNBS-induced rat model of inflammatory bowel disease. The therapeutic effect of 5-ASA on intestinal inflammation may be through the inhibition of TL1A and NF-κB.