论文部分内容阅读
用低频功率超声波辐照方法，实现了在低浓度变性剂（４ｍｏｌ／Ｌ尿素）中对大肠杆菌体系表达产物缩短的人巨噬细胞集落刺激因子（ｈＭ－ＣＳＦ）包涵体的解聚，以期改进普遍采用的强烈变性剂（８ｍｏｌ／Ｌ尿素）溶解包涵体方法，超声处理后的ＳＤＳ－ＰＡＧＥ在分子量１７０００上显示了与８ｍｏｌ／Ｌ尿素溶解结果相同的条带．表现出功率超声方法在基因工程包涵体解聚中的应用前景．
Using low-frequency power ultrasonic irradiation method, depolymerization of human macrophage colony-stimulating factor (hM-CSF) inclusion bodies shortened by the expression product of E. coli in low concentration denaturant (4 mol / L urea) The widely used method of solubilization of inclusion bodies with a strong denaturing agent (8 mol / L urea) shows that SDS-PAGE after sonicating shows the same band as that of 8 mol / L urea at a molecular weight of 17000. It shows the prospect of power ultrasound in the depolymerization of genetic engineering inclusion bodies.