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目的探索轻度苯中毒者血小板线粒体基因9个碱基(9bp)序列基因多态性变化规律。方法选取27例轻度苯中毒者作为病例组,40例健康检验者作为对照组。抽取其肘部静脉血,乙二胺四乙酸抗凝,分离纯化血小板然后进行血小板计数并提取脱氧核糖核酸,聚合酶链式反应扩增线粒体基因,测序并分析其基因9bp基因多态性频率。结果轻度苯中毒者血小板线粒体基因9bp的删除基因型频率(33.3%)高于对照组(10.0%),差异有统计学意义(χ2=5.090,P=0.024),OR值(95%CI)为4.5(1.21~16.62)。结论说明线粒体基因组9bp序列删除基因型可能是苯中毒的风险基因之一,携带删除基因型的个体苯中毒发生风险增高。
Objective To explore the polymorphism of 9 bp gene sequence of platelet mitochondrial DNA in mild benzene poisoning. Methods Twenty-seven patients with mild benzene poisoning were selected as the case group and 40 healthy persons as the control group. The venous blood of the elbow, anticoagulant of ethylenediaminetetraacetic acid (ACP), platelet count, platelet count and DNA extraction were extracted and the mitochondrial DNA was amplified by polymerase chain reaction (PCR). The 9bp gene polymorphism was sequenced and analyzed. Results The genotype frequency of platelet mitochondrial 9bp deletion was 33.3% higher than that of the control group (10.0%) (χ2 = 5.090, P = 0.024) and OR (95% CI) 4.5 (1.21 ~ 16.62). The results suggest that the mitochondrial genome 9bp deletion genotype may be one of the risk factors of benzene poisoning, and individuals with deletion genotypes have an increased risk of benzene poisoning.