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目的 研究视黄酸 (RA)促进脐血淋巴细胞 (CBL)细胞因子表达的作用途径 ,探讨视黄酸受体α在该过程中的可能作用。方法 收集 19例健康新生儿的脐血标本进行体外培养 ,采用RT PCR法检测CBL中视黄酸受体α(RAR α)、IL 2、IFN γ和IL 4的mRNA水平。结果 RA上调CBL中RAR α的基因表达水平 ,培养 2 4小时时的上调幅度为 (1 17± 0 0 7)倍 (P <0 0 0 1)。RA上调IL 2的基因表达水平 ,培养 2 4小时时的上调幅度为 (1 2 9± 0 2 0 )倍 (P <0 0 0 1)。RA也上调IFN γ的基因表达水平 ,培养 4 8小时时的上调幅度为 (1 39± 0 2 6 )倍 (P <0 0 0 1)。RA还上调IL 4的基因表达水平 ,培养 2 4小时时的上调幅度为 (1 5 2± 0 4 4 )倍 (P <0 0 0 2 )。RA使RAR α基因表达的上调幅度与RA使IL 2、IFN γ和IL 4基因表达的上调幅度皆存在正相关 ,r值分别为 0 4 0、0 39和 0 4 6 ,(P <0 0 5 )。结论 RAR α可能参与RA对CBL细胞因子 (IL 2、IFN γ和IL 4 )表达的促进作用。RA可能通过增加RAR α的表达而上调IL 2、IFN γ及IL 4等细胞因子的表达 ,从而促进CBL的功能
Objective To study the mechanism of retinoic acid (RA) promoting the expression of cytokines in cord blood lymphocytes (CBL) and to explore the possible role of retinoic acid receptor α in this process. Methods Cord blood samples from 19 healthy newborns were collected and cultured in vitro. The mRNA levels of retinoic acid receptor α (RAR α), IL 2, IFN γ and IL 4 in CBL were detected by RT PCR. Results RA up-regulated the gene expression level of RARα in CBL, and the up-regulation of RAR α was (1 17 ± 0 0 7) times (P 0 01 0) at 24 hours. RA up-regulated the gene expression level of IL-2, and the up-regulation of IL-2 at 24 hours of culture was (12 9 ± 0 2 0) times (P 0 01). RA also up-regulated the gene expression of IFN-γ, up-regulated by (399 ± 0 2 6) -fold (P 0 001) at 48 hours. RA also up-regulated the gene expression level of IL-4, up-regulated by (14 ± 2) 4 times (P <0 0 0 2) at 24 hours. RA upregulated the RAR α gene expression in a dose-dependent manner with RA up-regulation of IL 2, IFN γ and IL 4 gene expression, with r values of 0 40,09 and 0 46, respectively (P <0 0 5). Conclusion RAR α may be involved in the promotion of the expression of cytokines (IL 2, IFN γ and IL 4) in CBL. RA may up-regulate the expression of IL-2, IFN-γ and IL-4 and other cytokines by increasing the expression of RARα, thereby promoting the function of CBL