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应用 NADPH-d组织化学方法观察了大鼠第三脑室一氧化氮合酶阳性触液神经元的分布及其形态 ,并结合免疫组织化学技术研究了一氧化氮合酶与催产素在第三脑室触液神经元内的共存。结果显示 :在视前区至室间核后大细胞亚核平面的第三脑室室壁均有一氧化氮合酶阳性触液神经元分布 ,呈自前向后 ,由腹侧部渐向背侧部过渡迁移的特征。一氧化氮合酶阳性触液神经元绝大部分为大细胞型标记神经元 ,胞体呈卵圆形、梭形、多角形与倒置梨形。它们位于脑室管膜内、室管膜下 ,或距室管膜有一定距离处 ,但有突起伸至第三脑室。第三脑室催产素免疫阳性触液神经元的形态及分布与一氧化氮合酶阳性触液神经元基本相似 ,两者高度共存。催产素 /一氧化氮合酶双标神经元 ,约占阳性细胞总数的 90 .9%。上述三种阳性触液神经元与邻近核团关系密切 ,尤其与室旁核之间有很多的阳性纤维互相交错。本研究结果表明 ,第三脑室有大量的一氧化氮合酶与催产素免疫阳性神经元分布 ,并且高度共存 ,从而建立了催产素的下丘脑 -脑脊液 -垂体神经体液调节环路的结构基础 ,对生殖与性行为起着重要的调控作用
NADPH-d histochemical method was used to observe the distribution and morphology of synaptophysin-positive synaptic neurons in the third ventricle in rats. Combined with immunohistochemistry, the effects of nitric oxide synthase Coexistence within the fluid neurons. The results showed that in the preoptic area to the interventricular nucleus, the distribution of nitric oxide synthase positive synaptic neurons in the third ventricle in the subnuclear plane of the posterior macrocycle was changed from ventral to dorsal Migration characteristics. Nitric oxide synthase positive synaptic neurons for the vast majority of large cell labeled neurons, the body was oval, spindle, polygon and inverted pear-shaped. They are located in the ependymal, subependymal, or at a distance from the ependymal tube, but with protrusions that reach into the third ventricle. The morphology and distribution of oxytocin-immunostimulatory neurons in the third ventricle were similar to those of neurotrophic neurons positive for nitric oxide synthase, both of which were highly coexistent. Oxytocin / nitric oxide synthase double-labeled neurons, accounting for 90.9% of the total number of positive cells. The three positive palpable neurons are closely related to the adjacent nuclei. In particular, there are many positive fibers interlaced with the paraventricular nucleus. The results of this study show that the third ventricle has a large number of nitric oxide synthase and oxytocin-immunopositive neurons distribution, and a high degree of coexistence, which established the hypothalamic - cerebrospinal fluid - pituitary neurotransmitter loop structure basis, Reproductive and sexual behavior plays an important regulatory role