transgen相关论文
The integration characteristics of the exogenous growth hormone gene in a transgenic common carp （Cy
在转基因的动物的外长的基因的基因稳定性和表示效率是仔细，与集成有关，地点和拷贝数。在我们的实验室，由转基因的操作和随后的测试十......
Neurofibroma cell lines derived from mice transgenic for HTLV-I LTR tax express high levels of HTLV-I tax mRNA and prote......
Background The multidrug resistance(MDR)associated with the expression of the mdr1 gene and its productP-glycoprotein i......
Obtained transgenic wheat expressing pac1 mediated by Agrobacterium is resistant against Barley yell
In fission yeast (Schizosaccharomyces pombe), pac1 gene was cloned with 99.3% nucleo- tide sequence similarity with publ......
Fitness costs of reflex bleeding in the ladybird beetle Harmonia axyridis: the role of parental effe
Reflex bleeding is an important antipredator defense mechanism in Coccinellidae.We examined the costs of reflex bleeding......
The full length osRACD cDNA sequence was subcloned into the pBI121 plasmid in the antisense orientation under the contr......
Phytosulfokine-α(PSK-α), a biologically active peptide acting as a growth factor, plays a key role in cellular differe......
An experiment was conducted to compare the mRNA expression difference in rice leaves and roots under drought stress and ......
A herbicide safener AD-67 inducible cDNA was identified in an indica rice variety 9311 by mRNA differential display. The......
Using the Phosphomannose Isomerase (PMI) Gene from Saccharomyces cerevisiae for Selection in Rice Tr
The phosphomannose isomerase (PMI) gene from Saccharomyces cerevisiae acted as selectable marker and mannose acted as se......
Expression of auxin synthesis gene tms1 under control of tuber-specific promoter enhances potato tub
Phytohormones, auxins in particular, play an important role in plant development and productivity.Earlier data showed po......
Novel promoters that confer root-specific expression would be useful for engineering resistance against problems of nutr......
Infection of Early and Young Callus Tissues of Indica Rice BPT 5204 Enhances Regeneration and Transf
该文从挂篮荷载计算、施工流程、支座及临时固结施工、挂篮安装及试验、合拢段施工、模板制作安装、钢筋安装、混凝土的浇筑及养生......
Optimization ofAgrobacterium tumefaciens-Mediated Immature Embryo Transformation System and Transfor
该文从挂篮荷载计算、施工流程、支座及临时固结施工、挂篮安装及试验、合拢段施工、模板制作安装、钢筋安装、混凝土的浇筑及养生......
Infection of Early and Young Callus Tissues of Indica Rice BPT 5204 Enhances Regeneration and Transf
A rapid and reproducible method to develop transgenic plants with enhanced transformation efficiency using Agrobacterium......
Optimization ofAgrobacterium tumefaciens-Mediated Immature Embryo Transformation System and Transfor
Since maize is one of the most important cereal crops in the world, establishment of an efifcient genetic transformation......
Polyclonal antibody against an insect excitatory toxin BmKIT from Buthus Martensii karsch and detect
An insect excitatory toxin gene from Buthus martensii Karsch (BmKIT) was cloned into the expression vector, pET-28a. BmK......
Elevated expression of the rotavirus VP6 antigen in transgenic plants is a critical factor in the development of a safe ......
运用基因组步移技术分离获得了CaKR1基因上游-1594 bp的启动子序列，命名为CaKR1p，发现其中含有SA、ABA、低温等信号应答原件以及其它......
草甘膦是世界上应用最广泛的广谱性除草剂,目前我国还没有自主知识产权的抗草甘膦油菜品种。本研究利用农杆菌介导的油菜下胚轴遗......
为研究天然植物激素脱落酸（ABA）作为转基因植物选择性除草剂以实现环境友好型除草策略的可能性,采用改变拟南芥SOAR1基因表达的方法......
为探明ZmCBL1和ZmCBL9在玉米中的表达调控及功能的差异,克隆了ZmCBL1和ZmCBL9的启动子区域。PlantCARE分析显示,2个启动子中含有多......
通过C4转基因技术改善C3作物光合作用,以期提高作物产量是国内外研究的热点之一。然而,目前关于转磷酸烯醇式丙酮酸羧化酶（PEPC）基因......
Using linker peptide LP4/2A for multiple gene transformation is considered to be an effective method to stack or pyramid......
位点特异性重组系统能够在植物遗传转化中更准确、可靠的操纵外源DNA的引入或删除，已成为植物遗传操作中的重要工具。本文简要介绍......
[目的]探讨取食转基因棉棉叶的白粉虱对T纹豹蛛生长发育及捕食的影响。[方法]在室内条件下,测定取食转基因棉和常规棉的白粉虱对T......
棉花抗虫核不育系的培育研究＊靖深蓉邢朝柱袁有禄刘少林郭立平王海林（中国农业科学院棉花研究所，河南安阳４５５１１２）ＤｅｖｅｌｏｐｍｅｎｔｏｆＮｕｃｌｅａｒＭａｌｅＳｔｅｒｉｌｅＬｉｎｅｓｏｆＵｐｌａｎｄＣｏｔｏｎＲｅｓｉｓｔａｎｔｔｏＣｏｔｏｎＢｏｌ.........
Changes in skin levels of two neutotrophins （glial cell line derived neurotrohic factor and neurotro
Neurotrophins are important for the development and maintenance of both high and low threshold mechanoreceptors(HTMRs an......
目的构建转基因枸杞表达系统,并表达HIV壳体蛋白.方法以重组质粒pET-3a-MA4-CA为模板,PCR扩增MA4-CA基因,克隆至pGEM-T质粒中,转化......
目的探讨不同表达量的热休克蛋白27（Hsp27）在小鼠心脏中的作用。方法构建在心肌中特异性表达Hsp27的不同表达量的转基因鼠（TG），对照组为......
运用基因组步移技术分离获得了CaKR1基因上游-1594bp的启动子序列，命名为CaKR1p，发现其中含有SA、ABA、低温等信号应答原件以及其它......
目的:观察阿尔茨海默病(AD)的相关基因p35nck5a 基因5′侧翼区指导报告基因表达的能力.方法:将对本实验室所克隆的p35nck5a基因5′......
用低能氩离子束介导将改良的绿色荧光蛋白基因（ｍＧＦＰ４）导入小麦栽培品种皖９２１０和皖麦３２号的成熟胚细胞。在含有１００￣１４０ｍ／Ｌ巴龙霉素培养基上继代培养后，获得了......
利用花粉管通道法,用含抗除草剂基因bar及抗虫基因pin2的质粒pTW-α转化中籼HD357.经用除草剂Basta进行筛选,得到5株抗性苗.对其进......
Bicoid 是调控果蝇胚胎极性及以后分节发生过程的重要基因，这一基因编码的蛋白是一种转录调控因子，对一组早期胚胎发育过程中的多齿......
草甘膦(N-phosphonomethyl-glycine, glyphosate)毒性作用机理是竞争性抑制莽草酸途径中的5-烯醇丙酮莽草酸-3-磷酸合成酶(5-enolp......
目的探讨携带自杀基因(TK)的溶瘤腺病毒(Ad-ETK)治疗实体肿瘤患者的安全性及临床疗效。方法选择晚期实体瘤常规治疗无效或已无法实......
