LENTIVIRAL相关论文
针对绵羊Myostatin基因特异性序列,设计4对shRNA,并合成高表达载体。将干扰质粒和高表达载体瞬时共转染HEK293细胞,应用Real-time ......
目的:构建携带人环核苷酸门控离子通道基因(HCN4)、发育调控相关转录因子基因(Tbx3)及增强型绿色荧光蛋白基因(EGFP)的慢病毒载体,......
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目的利用慢病毒介导的RNA干扰技术构建人淋巴母细胞TK6细胞的PARP-1基因沉默细胞株,为后续PARP-1基因功能和表观遗传学调控机制的......
目的 构建携带胞嘧啶脱氨酶(CD)和血管内皮抑素(ES)基因的慢病毒载体.方法 根据GenBank提供大肠杆菌源CD和ES基因序列设计、合成2......
目的::探讨表达猪H1N1亚型流感病毒三聚体HA的重组慢病毒(rLV-HA-GCN4)对BALB/c小鼠的免疫保护效果。方法:将雌性BALB/c小鼠随机分为......
目的:构建人免疫缺陷病毒(HIV)假病毒模型,用多种HIV逆转录酶和蛋白酶抑制剂作用于该模型,以检测其是否能有效用于HIV抑制药物的筛选......
目的:通过慢病毒载体构建过表达SND1的OVCAR3稳定的表达株。方法:重组的pLVX-FLAG-SND1表达载体与包装质粒共同转染293T细胞,获得携......
目的:应用慢病毒介导的RNA干扰技术,建立稳定表达siPin1的鼻咽癌细胞株。方法:将对照组病毒液lv-3和实验组病毒液siPin1感染CNE2细胞......
AIM: To evaluate the influence of E2F-1 on the growth of human gastric cancer(GC) cells in vivo and the mechanism involv......
AIM: To investigate the targeted inhibition of proliferation and migration of SW620 human colon cancer cells by upregula......
目的应用RNA沉默技术下调前列腺癌CWR22RV1细胞株的Rictor基因表达,并筛选具有稳定转染Rictor-shRNA的细胞株,为进一步研究Rictor......
目的构建慢病毒载体将Foxq1干扰序列递达到人肝癌7721细胞中,观察该细胞中Foxq1的表达并评估慢病毒载体的有效性。方法体外合成与筛......
目的观察慢病毒载体介导的增强型绿色荧光蛋白(LV-EGFP)经不同浓度和途径转染大鼠角膜的转染效率及毒性,探讨慢病毒转染角膜的最佳途......
目的:构建金黄色葡萄球菌肠毒素C3(SEC3)过表达慢病毒载体并体外检测其表达目的基因。方法:聚合酶链反应(PCR)扩增SEC3基因片段;用Age I......
目的构建PRL-3基因RNA干扰慢病毒载体并建立被稳定感染的HepG2单克隆细胞株。方法设计PRL-3基因特异性RNAi靶序列,合成靶序列的DNA......
背景与目的:维甲酸诱导基因G(retinoic acid-induced gene G,RIG-G)是从急性早幼粒细胞性白血病细胞系NB4细胞中克隆出的肿瘤抑制基因......
Effects of Over-expression of ANXA10 Gene on Proliferation and Apoptosis of Hepatocellular Carcinoma
The effects of over-expression of ANXA10 gene on proliferation and apoptosis of hepatocellular carcinoma cell line HepG2......
目的构建大鼠整合素连接激酶-RNAi(ILK-RNAi)慢病毒表达载体,为下一步干预研究细胞转分化及其信号通路奠定基础。方法针对大鼠ILK基......
目的:构建携载人白介素-8(IL-8)shRNA的慢病毒并鉴定其对MDA-MB-231细胞该基因的沉默效率。方法:设计两个IL-8基因特异性siRNA靶点,与pM......
【目的】探讨肝再生磷酸酶-3(PRL-3)的表达对肝癌细胞侵袭能力的影响。【方法】设计、合成4对针对PRL-3基因siRNA的寡核苷酸序列,分......
目的探讨下调claudin-1对胆囊癌细胞生物学行为的影响。方法通过慢病毒LV-CLDN1-RNAi感染下调claudin-1在胆囊癌细胞SGC996中的表......
【目的】筛选高效的foxp3 RNA干扰的靶点用于阻断CD4^+CD25^+ Treg细胞的免疫抑制功能。【方法】使用oligoengine软件设计foxp3基因s......
AIM: To test whether the replication of human rhinovirus(HRV) is regulated by micro RNAs in human bronchial epithelial c......
目的 通过体外实验探讨过表达SEMA3G对人胰腺癌细胞PANC-1细胞增殖、侵袭和迁移能力的影响。方法将携带SEMA3G的慢病毒载体转染人......
Leukemogenic Fusion Gene (p190 BCR-ABL) Transduction into Hematopoietic Stem/Progenitor Cells in the
Patients with Philadelphia chromosome (p190 BCR-ABL fusion gene)-positive acute lymphoblastic leukemia have a poor progn......
目的探讨Foxp1对肝癌细胞增殖、凋亡和迁移的影响。方法体外合成干扰Foxp1功能的小核酸片段(siRNA Foxp1),通过重组技术将其插入到......
Combined antiviral activity of interferon-α and RNA interference directed against hepatitis C withou
The current standard interferon-alpha(IFN-α)-based therapy for chronic hepatitis C virus(HCV) infection is only effecti......
Differentiation Character of Adult Mesenchymal Stem Cells and Transfection of MSCs with Lentiviral V
This study examined the differentiation character and pluripotency of mesenchymal stem cells (MSCs) under different cond......
Construction of a lentiviral vector over - expressing BDNF and its transfection into adipose derived
Objective:To construct a recombinant lentiviral vector that co-express ZsGreen and BDNF and establish an Adipose derived......
目的:1.观察高糖对H9c2心肌细胞增殖、凋亡及纤维化的影响。2.观察以Notch-1为靶点干预对高糖诱导的H9c2心肌细胞增殖、凋亡及纤维......
Expression of human adrenomedullin gene in gastric adenocarcinoma and construction and identificatio
Background:To explore the expression of adrenomedullin in gastric adenocarcinoma tissues,and to construct a eukaryotic e......