论文部分内容阅读
目的探讨吡咯里西啶生物碱Senkirkine对黑色素瘤细胞B-16生长的抑制作用及细胞内谷胱甘肽含量、谷胱甘肽过氧化物酶(Glutathione Peroxidase,GPx)以及谷胱甘肽还原酶(Glutathione Reductase,GR)活性的影响。方法以黑色素瘤B-16细胞为实验模型,采用MTT法和DTNB法检测Senkirkine与B-16细胞孵育后对B-16细胞存活率的影响,对细胞内还原型谷胱甘肽(GSH)、氧化型谷胱甘肽(GSSG)含量,GSH/GSSG比率的影响,采用DTNB和NADPH分光光度法检测GPx以及GR的活性。结果Senkirkine可以显著抑制B-16细胞的增殖(P<0.001),同时明显降低了细胞内GSH的含量以及GSH/GSSG的比例,并呈时间、剂量依赖性(P<0.001);同时GPx活力与对照组相比显著提高(P<0.05)而GR活力没有显著性的改变。结论在本试验条件下,Senkirkine能够抑制B-16黑色素瘤细胞的增殖,同时降低了B-16细胞抗氧化应激损伤的能力。
Objective To investigate the inhibitory effect of pyrrolizidine alkaloid Senkirkine on the growth of melanoma B-16 cells and the effects of glutathione, glutathione peroxidase (GPx) and glutathione reductase (Glutathione Reductase, GR) activity. Methods The effects of Senkirkine and B-16 cells on the survival rate of B-16 cells were detected by MTT assay and DTNB assay. The effects of GSH, Oxidized glutathione (GSSG) content and GSH / GSSG ratio. The activities of GPx and GR were measured by DTNB and NADPH spectrophotometry. Results Senkirkine could significantly inhibit the proliferation of B-16 cells (P <0.001) and significantly reduce the content of intracellular GSH and GSH / GSSG in a time-and dose-dependent manner (P <0.001) Compared with control group, the activity of GR increased significantly (P <0.05) and no significant change of GR activity. Conclusions Under this test condition, Senkirkine can inhibit the proliferation of B-16 melanoma cells and reduce the ability of B-16 cells to resist oxidative stress.