论文部分内容阅读
目的:采用血清药理学方法探讨冠心苏合胶囊对过氧化氢(H2O2)致心肌细胞过氧化损伤的保护作用。方法:血清药理学方法制备含药血清(0.8g生药/kg),体外培养原代乳鼠心肌细胞,随机分为正常对照组、空白血清对照组、H2O2损伤模型组、冠心苏合低、中、高剂量(5%,7.5%,10%)干预组,建立H2O2氧化损伤模型,以不同剂量的冠心苏合含药血清进行干预。MTT法测定细胞存活率,并检测各组心肌细胞培养上清液中乳酸脱氢酶(LDH)、丙二醛(MDA)的含量及总超氧化物歧化酶(T-SOD)及过氧化氢酶(CAT)的活性。结果:100μmol/L H2O2处理2h,原代乳鼠心肌细胞存活程度适中,模型制备的重复性较好;与H2O2单纯损伤组和空白血清对照组比较,冠心苏合低、中、高(5%,7.5%,10%)剂量干预组的心肌细胞有较高存活率(P<0.01),且呈现一定的剂量依赖性;与正常细胞相比,H2O2损伤后MDA、LDH含量明显升高,T-SOD、CAT活性降低,而冠心苏合含药血清可剂量依赖性的降低损伤细胞MDA、LDH含量,升高CAT、T-SOD活性,结果具有显著统计学差异。结论:冠心苏合胶囊能够提高H2O2诱导的原代乳鼠心肌细胞存活率;下调MDA、LDH水平,上调CAT、SOD水平,对氧化损伤的心肌细胞起保护作用
Objective: To explore the protective effect of Guanxin Suhe Capsule on peroxidation injury of cardiomyocytes induced by hydrogen peroxide (H2O2) by serum pharmacology. Methods: Serum pharmacological methods were used to prepare serum containing 0.8g crude drug / kg. Primary neonatal rat cardiomyocytes were cultured in vitro and randomly divided into normal control group, blank serum control group, H2O2 injury model group, Medium and high dose (5%, 7.5%, 10%) intervention group, the establishment of H2O2 oxidative damage model, with different doses of Guanxin Suhe serum containing intervention. Cell viability was measured by MTT assay. The levels of lactate dehydrogenase (LDH), malondialdehyde (MDA) and total superoxide dismutase (T-SOD) and hydrogen peroxide Enzyme (CAT) activity. Results: After treated with 100μmol / L H 2 O 2 for 2h, the viability of primary neonatal rat cardiomyocytes was moderate and the reproducibility of the model preparation was better. Compared with the H 2 O 2 -induced injury group and the blank serum control group, (P <0.01), and showed a dose-dependent manner. Compared with normal cells, the content of MDA and LDH in H2O2-induced injury increased significantly, T-SOD and CAT decreased. However, Guanxin Suhe containing serum could decrease the content of MDA and LDH and increase the activity of CAT and T-SOD in injured cells in a dose-dependent manner. The results showed that there was significant difference. CONCLUSION: Guanxin Suhe Capsule can improve the survival rate of neonatal rat cardiomyocytes induced by H2O2; down-regulate the levels of MDA and LDH; up-regulate the levels of CAT and SOD; and protect the oxidative injured cardiomyocytes