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以荧光探针Hoechst33342（Ho3334）特异性标记大白鼠精子和肝细胞DNA，用科学级冷却型CCD显微荧光图像系统获取单个细胞的荧光图像，再以图像处理软件“Image-ProPlus”，分析测量各细胞的DNA相对含量和细胞核的相对面积．实验数据表明：鼠肝细胞的DNA相对含量分别为精子的DNA相对含量的2，4，8倍，符合生物学的规律，说明这套系统的定量结果是可靠的．
Fluorescent probe Hoechst33342 (Ho3334) specific labeling rat sperm and hepatocyte DNA, with a scientific-class cooling CCD microscopic fluorescence imaging system to obtain a single cell fluorescence images, and then image processing software “Image-ProPlus”, analysis and measurement The relative content of DNA in each cell and the relative area of the nucleus. Experimental data show that: the relative content of DNA in rat liver cells were 2,4,8 times the relative content of sperm DNA, in line with the laws of biology, indicating that the quantitative results of this system is reliable.