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(Ca、Mg)-ATP 酶是细胞膜上重要的酶,它是钙泵的一部分,将细胞内的钙离子主动转运至细胞外。近年来发现,患某些疾病时,患者此酶的活力发生一定的改变,为了探讨某些疾病的发病机理,我们建立了人红细胞膜(Ca、Mg)-ATP 酶测定方法,并进行了临床初步应用。材料与方法一、红细胞膜的制备:取抗凝血2ml,离心去血浆,红细胞用等渗溶液洗三次,再加入预冷的10mMTris-HCl 缓冲液,使红细胞溶血,离心去血红蛋白,粉红色的膜再用同样缓冲液洗三次,得到乳白色红细胞膜,分装,放-50℃冰箱备用。
(Ca, Mg) -ATPase is an important enzyme in the cell membrane. It is part of the calcium pump that actively transports intracellular calcium ions to the outside of the cell. In recent years, found that patients suffering from certain diseases, the enzyme activity of patients with some changes, in order to explore the pathogenesis of certain diseases, we established a human erythrocyte membrane (Ca, Mg) -ATP enzyme assay, and clinical Preliminary application. Materials and methods First, the preparation of erythrocyte membrane: take anticoagulant blood 2ml, centrifuged to the plasma, red blood cells were washed three times with isotonic solution, then add pre-cooled 10mMTris-HCl buffer, hemolysis of erythrocytes, centrifugation to hemoglobin, pink The membrane was washed three times with the same buffer to obtain a milky white erythrocyte membrane, aliquoted and placed in a -50 ° C refrigerator.