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为了从成熟红麻叶片中提取高质量、高产量的基因组DNA,针对红麻成熟叶片中多糖、多酚含量较高的特性,利用改良CTAB法及改良SDS法分别提取红麻品种福红952成熟叶基因组DNA,并通过琼脂糖凝胶电泳和紫外分光光度计测定进行DNA质量检测。结果表明:改良CTAB法提取的基因组DNA电泳时点样孔干净,条带整齐无拖带,OD260/OD280为1.9左右,产率可达1.84μg/g,其质量、产量都高于改良SDS法,所提取的DNA可用于红麻RAPD分子标记、线粒体DNA、叶绿体DNA通用引物PCR扩增。改良CTAB法是提取成熟红麻叶片DNA的有效方法,并且可用于红麻分子标记及胞质基因组学研究。
In order to extract high-quality and high-yielding genomic DNA from mature kenaf leaves, according to the characteristics of polysaccharides and polyphenols in mature leaves of kenaf, the kenaf varieties Fuhong 952 were extracted by modified CTAB method and modified SDS method Leaf genomic DNA and DNA quality testing by agarose gel electrophoresis and UV spectrophotometer. The results showed that the modified CTAB method showed that the spots were clean, the bands were neat and tidy, the OD260 / OD280 was about 1.9, and the yield was up to 1.84 μg / g. The quality and yield of genomic DNA were all higher than that of the modified SDS method. The extracted DNA can be used for kenaf RAPD molecular marker, mitochondrial DNA, chloroplast DNA universal primer PCR amplification. Improved CTAB method is an effective method for extracting DNA from mature kenaf leaves, and can be used for molecular marker and cytoplasmic genomic study of kenaf.