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试验用SSR分子标记对60份宁夏粳稻种质资源进行遗传多样性分析。103对SSR引物表现多态性的有58对,共扩增出212条多态性条带,等位变异范围为2～9,平均每对引物3.7个;多态性信息含量(PIC)变幅为0.032～0.788,平均为0.403;高多态性位点主要发生在3号、6号和11号染色体上,而无多态性或低多态性位点主要发生在1号和10号染色体上;成对供试材料的遗传相似系数GS值变幅为0.642～0.958,平均为0.790,单个供试材料的平均GS值变幅为0.710～0.816,平均为0.781,亲缘关系较近;UPGMA聚类表明,在遗传相似系数约0.785处,供试材料可被分为11类,大部分材料被聚在一类中。
The SSR markers were used to analyze the genetic diversity of 60 Ningxia japonica germplasms. A total of 103 pairs of SSR primers showed 58 pairs of polymorphisms. A total of 212 polymorphic bands were amplified with an allelic variation ranging from 2 to 9 with an average of 3.7 pairs per primer. Polymorphism information content (PIC) With a range of 0.032-0.788, with an average of 0.403. High polymorphic loci mainly occurred on chromosomes 3, 6 and 11, but no polymorphism or low polymorphism occurred mainly on chromosomes 1 and 10 On the chromosomes, the genetic similarity coefficients of paired cultivars ranged from 0.642 to 0.958 with an average of 0.790. The average GS values of single test materials ranged from 0.710 to 0.816 with an average of 0.781. Clustering showed that at the genetic similarity coefficient of about 0.785, the test materials could be classified into 11 categories, most of which were clustered in one category.