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目的制备紫杉醇脂质体和紫杉醇冻干脂质体,并对其冻干前后的性质进行比较。方法采用薄膜分散法制备紫杉醇脂质体,用冷冻干燥技术将其冻干并进行形态和粒径的比较。采用质量分数5%的TritonX-100乙醇溶液对冻干前后的紫杉醇脂质体进行破乳测定含量,用Sephadex G-50分离紫杉醇脂质体和游离药物。结果冻干前紫杉醇脂质体的平均粒径为210 nm,冻干后脂质体的平均粒径为279 nm。冻干前后脂质体的含量和包封率变化不大,离心加速试验的结果也没有明显区别。结论采用薄膜分散-超声法能够制备出包封率较高、粒径比较均匀的脂质体,经冷冻干燥后,其各项性质没有明显的变化。
Objective To prepare paclitaxel liposomes and paclitaxel lyophilized liposomes, and compare their properties before and after lyophilization. Methods Paclitaxel liposomes were prepared by the thin film dispersion method. The liposomes were lyophilized by freeze - drying technique and their morphology and particle size were compared. Paclitaxel liposomes were lyophilized before and after lyophilization using TritonX-100 ethanol solution at a mass fraction of 5%, and paclitaxel liposomes and free drug were separated by Sephadex G-50. Results The average particle size of paclitaxel liposomes before lyophilization was 210 nm, and the average size of liposomes after lyophilization was 279 nm. The content and entrapment efficiency of the liposomes before and after lyophilization did not change much, and there was no obvious difference between the results of centrifugal acceleration test. Conclusion The liposomes with high encapsulation efficiency and relatively uniform particle size can be prepared by the membrane dispersion - ultrasonic method. After freeze - drying, the properties of the liposomes have no obvious changes.