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金龟子绿僵菌是昆虫种群自然控制和害虫生物防治中重要的虫生真菌,已被作为化学农药的替代品广泛应用于农林害虫的防治。其寄主范围和地理分布极广,因此种群结构也十分复杂。为明确安徽省土栖金龟子绿僵菌的种群遗传结构,采用ISSR分子标记技术对采集自安徽省不同地区土壤中的116株金龟子绿僵菌进行遗传异质性分析。分子数据显示,筛选出的8个引物共获得79个位点,其中多态位点比率为100%。不同地区种群的Nei’s基因多样性(H)为0.2796,Shannon信息指数(I)为0.4425,种群间存在一定程度的基因流(Nm=4.4282)和遗传分化(Gst=0.1015),种群内的基因多样度占总居群的89.85%,种群间占10.15%,表明安徽土栖金龟子绿僵菌的遗传变异主要来源于种群内,且种群内表现出较大的遗传变异。并采用UPGMA对所有供试材料进行聚类分析,得到7大类种群。结果表明,安徽土栖金龟子绿僵菌之间的亲缘关系与地理来源不存在相关性。
Metarhizium anisopliae is an important entomogenous fungus in the natural control of insect population and biological control of pests and has been widely used as a substitute for chemical pesticides in the prevention and control of agricultural and forestry pests. Its host range and geographical distribution is very wide, so the population structure is also very complicated. In order to clarify the population genetic structure of Metarhizium anisopliae in Anhui Province, ISSR molecular markers were used to analyze the genetic heterogeneity of 116 M. scotch Metarhizium strains collected from soil in different areas of Anhui Province. Molecular data showed that a total of 79 loci were obtained from the 8 primers selected, of which the percentage of polymorphic loci was 100%. The Nei’s gene diversity (0.2796) and Shannon’s information index (I) were 0.4425 in different regions, and there was a certain degree of gene flow (Nm = 4.4282) and genetic differentiation (Gst = 0.1015) Degrees accounted for 89.85% of the total population, accounting for 10.15% of the population, indicating that the genetic variation of Metarhizium anisopliae from Anhui Province mainly came from within the population, and the population showed large genetic variation. All the tested materials were clustered by UPGMA to get seven major populations. The results showed that there was no correlation between the genetic relationship and the geographical origin of Metarhizium anisopliae in Anhui Province.