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目的： 为了系统研究人胃癌基因组中Ｃｘ 基因的表达状况。方法： 本文采用Ｎｏｒｔｈｅｒｎ 印迹杂交和ＲＴＰＣＲ 方法， 对１５ 例人胃癌组织、配对癌旁组织、正常组织及人胃腺癌ＭＧＣ８０３ 细胞株的Ｃｘ２６ 、Ｃｘ３１－１ 、Ｃｘ３２ 、Ｃｘ３３、Ｃｘ３７ 、Ｃｘ４０、Ｃｘ４３ 、Ｃｘ４６ 八种Ｃｘ 基因表达进行了一系列检测。结果：发现在人正常胃粘膜上皮中高水平表达而在胃癌中表达极其微弱或根本无表达的细胞连接蛋白基因的表达规律，首次明确了Ｃｘ 基因在人胃癌基因组中的表达谱。其中人正常胃粘膜上皮细胞中Ｃｘ３２ 、Ｃｘ３７ 、Ｃｘ４３ 在ｍ ＲＮＡ 水平上有高水平表达； 与正常相反， 人胃癌细胞除Ｃｘ４３ 在ｍ ＲＮＡ 水平上有低水平表达外， 其他连接蛋白基因均无转录活性； 而人胃腺癌ＭＧＣ８０３ 细胞株中， Ｃｘ３７ 、Ｃｘ４６ 在ｍ ＲＮＡ 水平上有一定程度的表达。结论：本研究表明Ｃｘ３２ 可能是人胃上皮细胞基因组中维持细胞间隙连接通讯功能的特异表达的Ｃｘ 基因，Ｃｘ４６ 可能是胃癌Ｃｘ 基因表达的一种变异。Ｃｘ３７、Ｃｘ４３ 不是人胃上皮细胞的特异表达基因。本研究证实了Ｃｘ 基因在肿瘤细胞中表达下调，Ｃｘ 基因具有潜在的抑瘤基因的生物学活性。
Objective: To systematically study the expression of Cx gene in the human gastric cancer genome. Methods: Northern blot hybridization and RT-PCR were used in this study. Cx26, Cx31-1, Cx32, Cx33, Cx37, and Cx40 of 15 human gastric cancer tissues, matched cancer adjacent tissues, normal tissues, and human gastric adenocarcinoma MGC-803 cell lines were used. A series of tests were performed on the expression of eight Cx genes in Cx43 and Cx46. RESULTS: The expression pattern of the cell connexin gene was found in human normal gastric epithelium at high level but extremely weak or not at all in gastric carcinoma. The expression pattern of Cx gene in human gastric cancer genome was firstly defined. In normal human gastric epithelial cells, Cx32, Cx37, and Cx43 are highly expressed at the m RNA level. Contrary to normal, human gastric cancer cells have no transcription except that Cx43 has a low level of m RNA expression. Activity; In the human gastric adenocarcinoma MGC-803 cell line, Cx37 and Cx46 were expressed to a certain extent on m RNA level. Conclusion: This study suggests that Cx32 may be the specific expression of Cx gene in the genome of human gastric epithelial cells that maintains gap junctional communication. Cx46 may be a variant of Cx gene expression in gastric cancer. Cx37 and Cx43 are not specifically expressed genes in human gastric epithelial cells. This study confirmed the down-regulation of Cx genes in tumor cells. The Cx gene has potential biological activity of anti-tumor genes.