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目的探索一种简便、快速、稳定的羊水细胞培养和染色体制备的方法。方法2003年1月至2007年12月对785例16-30孕周有产前诊断指征的孕妇,抽取羊水,采取T型细胞瓶培养法T型,7-9d后用细胞刮刀分散细胞集落传代,换液培养1-2天,加入秋水仙素3h后,收获羊水细胞制片,G显带分析。结果785例羊水培养成功778例,成功率99%。平均每例分裂相>100个,培养时间8-13d,平均9.5d。结论该方法染色体分裂相多,细胞破坏小,染色体形态及分散性好,成功率高,出报告时间短等优点,对异常胎儿能及早终止妊娠,有实用推广价值。
Objective To explore a simple, rapid and stable amniotic fluid cell culture and chromosome preparation methods. Methods From January 2003 to December 2007, 785 pregnant women with prenatal diagnosis of 16-30 gestational weeks were enrolled. Taken together, T-cell flask culture method was adopted to take amniotic fluid. After 7-9 days, the cells were scattered with cell scraper Passage, liquid culture 1-2 days, after adding colchicine 3h, amniotic fluid cell harvest, G-banding analysis. Results 778 cases of 778 cases of amniotic fluid culture success rate of 99%. On average, each split phase> 100, the training time 8-13d, an average of 9.5d. Conclusion This method has many advantages, such as more chromosomes, fewer cell disruptions, better chromosomal morphology and dispersibility, higher success rate and shorter reporting time. It is valuable in popularizing fetal abnormalities as early as possible.