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目的揭示支气管哮喘患者异常黑胆质型与非异常黑胆质型的血浆代谢组变化情况及发生机制。方法对61例异常黑胆质型支气管哮喘患者和55名非异常黑胆质型支气管哮喘患者的血浆进行核磁共振氢谱检测,通过谱图分段积分后运用正交偏最小二乘判别分析法(OPLS-DA)分析所采集的图谱。结果异常黑胆质型哮喘患者与健康对照组相比,血浆中异亮氨酸、亮氨酸、丙氨酸、缬氨酸、酪氨酸、甘氨酸等氨基酸以及谷氨酰胺、α-葡萄糖、β-葡萄糖、糖蛋白、肌酸、乳酸、肉碱、丙酮、丙酮酸、脂类[包含低密度脂蛋白(LDL)和极低密度脂蛋白(VLDL)]、不饱和脂类的含量均有不同程度的降低(P<0.05)。与非异常黑胆质型哮喘患者相比,异常黑胆质型哮喘患者血浆中α-葡萄糖、β-葡萄糖、糖蛋白明显升高(P<0.05),丙氨酸、谷氨酸、缬氨酸、亮氨酸、异亮氨酸、甘氨酸、酪氨酸、苯丙氨酸、1-甲基组氨酸等多种氨基酸含量升高,乙酰乙酸、丙酮,丙酮酸、乳酸、肌酸、肌酸酐、肉碱等代谢物含量升高,LDL和VLDL、不饱和脂类、乙酸、乙酰半胱氨酸、谷氨酰胺、鲨肌醇等化合物含量升高(P<0.05)。结论异常黑胆质型哮喘患者体内能量代谢和蛋白质代谢发生改变,体内糖异生和免疫功能的紊乱程度较严重,这可能是异常黑胆质型哮喘形成的生物学基础之一,也从一个侧面证实维医“同病异证”的科学性。
Objective To reveal the changes of plasma metabolome in patients with asthenospermia and non-abnormal savda type in bronchial asthma and its mechanism. Methods The plasma of 61 patients with abnormal savda-type asthma and 55 patients with non-abnormal savda type bronchial asthma were detected by 1H nuclear magnetic resonance (1H-NMR) spectroscopy, and the results were analyzed by the method of orthogonal partial least squares discriminant analysis (OPLS-DA) analysis of the collected profiles. Results In patients with abnormal savda type asthma, compared with the healthy control group, amino acids such as isoleucine, leucine, alanine, valine, tyrosine and glycine, and glutamine, α-glucose, β-Glucose, Glycoprotein, Creatine, Lactic Acid, Carnitine, Acetone, Pyruvate, Lipids [including Low Density Lipoprotein (LDL) and Very Low Density Lipoprotein (VLDL) Varying degrees of reduction (P <0.05). Compared with patients with non-abnormal savda type asthma, the plasma levels of α-glucose, β-glucose and glycoprotein in patients with abnormal savda type asthma were significantly increased (P <0.05), and the contents of alanine, glutamic acid and valine Amino acids such as acid, leucine, isoleucine, glycine, tyrosine, phenylalanine, and 1-methylhistidine were increased, and the content of various amino acids such as acetoacetate, acetone, pyruvic acid, Creatinine, carnitine and other metabolites increased, LDL and VLDL, unsaturated lipids, acetic acid, acetylcysteine, glutamine, scyllo-inositol and other compounds increased (P <0.05). Conclusion Abnormal Savda type asthma patients with energy metabolism and protein metabolism change, the degree of in vivo gluconeogenesis and immune dysfunction more serious, which may be the biological basis for the formation of abnormal savda type asthma, but also from one Side of the confirmation of peacekeeping doctors “different syndromes ” scientific.