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目的探究人脐带血干细胞对肺纤维化大鼠TNF-α、NO的作用和影响。方法选取清洁并健康大鼠60只,随机分为治疗组与对照组各30只,两组大鼠先用博来霉素经气管注入的方法制造肺纤维化模型,治疗组大鼠在模型成功建立后注入干细胞,对照组大鼠不注入干细胞,此后在不同时间将大鼠处死,观察两组大鼠肺泡损伤程度及TNF-α、NO水平改变情况。结果对照组大鼠肺泡损伤程度比治疗组大鼠肺泡损伤严重,在肺纤维化程度方面0-1分之间例数两组大鼠相同,1.1-2分之间、2.1-3分之间治疗组大鼠例数较对照在大鼠少,此外在不同时间治疗组大鼠TNF-α、NO水平较对照组大鼠低,P<0.05,差异有统计学意义。结论将人脐带血内的干细胞注入肺纤维化大鼠体内后,随着时间的发展TNF-α、NO的水平有所降低,在一定程度上有保护性作用,TNF-α、NO的表达受到抑制,对大鼠肺纤维化有抑制作用。
Objective To investigate the effect of human umbilical cord blood stem cells on TNF-α and NO in pulmonary fibrosis rats. Methods Sixty clean and healthy rats were selected and randomly divided into treatment group and control group, 30 rats in each group. The rats in both groups were treated with bleomycin for tracheal injection to make pulmonary fibrosis model. The stem cells were injected into the stem cells and the control rats were not injected with stem cells. After that, the rats were sacrificed at different times and the changes of alveolar damage and the levels of TNF-α and NO in the two groups were observed. Results The alveolar damage in rats in the control group was more severe than that in the rats in the treatment group. The number of pulmonary fibrosis in the 0-1 group was the same between two groups of rats, between 1.1-2 and 2.1-3 The number of rats in the treatment group was less than that in the control group. In addition, the levels of TNF-α and NO in the treated group were lower than those in the control group at different time points (P <0.05), and the difference was statistically significant. Conclusion After the human cord blood stem cells were infused into pulmonary fibrosis rats, the levels of TNF-α and NO decreased with the time, and they had a protective effect on the expression of TNF-α and NO. Inhibition, inhibition of pulmonary fibrosis in rats.