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目的 :了解从西藏血蜱标本中分离的环状病毒 (Ti30 10 )的细胞生物学特性和敏感细胞范围 ,与国内其他地区环状病毒进行比较 ,为全面认识西藏地区环状病毒的特点和进一步确切分类提供依据。方法 :将病毒平行接种BHK13、Vero、C6/ 36、HeLa 4种细胞 ,逐日观察病毒的致细胞病变效应 (CPE) ,特异荧光发生部位和细胞数量 ,超薄切片电镜观察病毒的形态发生以及进行TCID50滴定。结果与结论 :Ti30 10环状病毒接种BHK13 细胞后第 3天产生CPE ,32 %～ 35%的细胞有病毒增殖 ,胞浆内检出特异荧光 ;电镜观察到胞浆内的病毒颗粒呈聚集状态 ,多呈晶格状排列 ,并具有病毒包涵体。TCID50 滴定在BHK13 细胞为 7.1,Vero为 5.8,C6/ 36和HeLa为 0。结果显示Ti30 10病毒与从鸟、鼠等标本中分离的Ti2 0 66和Ti2 0 76病毒一样 ,对BHK细胞敏感 ,产生CPE ;Vero细胞次之 ,对C6/ 36和HeLa细胞不敏感 ,在BHK13 细胞中上述 3株病毒具有相同的复制特点。结合其RNA基因组特点 ,该病毒同国内其他地区分离的环状病毒有较大差异 ,该差异显示了西藏地区特殊生态环境下环状病毒的独有性状。
OBJECTIVE: To understand the biological characteristics and sensitive cell range of the rotavirus (Ti30 10) isolated from the blood-swallow tick in Tibet and to compare with the other domestic circulating viruses. In order to fully understand the characteristics of the circulating virus in Tibet and further The exact classification provided the basis. Methods: Four strains of BHK13, Vero, C6 / 36 and HeLa cells were inoculated in parallel with each other. The cytopathic effect (CPE), specific fluorescent site and number of cells were observed daily. Morphogenesis and progression of the virus were observed by ultrathin electron microscopy TCID50 titration. RESULTS AND CONCLUSION: CPE was produced on the 3rd day after inoculating BHK13 cells with Ti30 10 and 32% ~ 35% of the cells proliferated with specific fluorescence. The cytoplasm virions were aggregated by electron microscopy , Mostly lattice-like arrangement, and has a virus inclusion body. TCID50 titrations were 7.1 for BHK13 cells, 5.8 for Vero, and 0 for C6 / 36 and HeLa. The results showed that Ti30 10 virus was sensitive to BHK cells, like Ti 2 0 66 and Ti 2 0 76 virus isolated from specimens such as birds and mice, and produced CPE. Vero cells were secondarily insensitive to C6 / 36 and HeLa cells, The above three viruses in the cell have the same replication characteristics. Combined with the characteristics of its RNA genome, the virus is quite different from that of other isolates in China. The difference shows the unique characteristics of the virus in Tibet in the special ecological environment.