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本文是1981年7月5~11日在波兰华沙召开的第6届国际原生动物学会时,Tra- ger教授在全体代表会议上所作的专题报告。本报告涉及近来关于寄生性原虫的培养工作。培养本身不是目的,而是达到其它目的一种方法。在恶性疟原虫培养之前,仅以人的感染和去脾的黑猩猩以及从1965年后新大陆夜猴的感染作为这种原虫的来源。现在我们已能使原虫在体外的人红细胞内连续不断的发育生长传代,使有可能在实验室内进行恶性疟原虫的分子生物学和细胞生物学的研究。通过培养,一般能得到更多的原虫,对于某些生化研究和免疫学及血清学研究都是大有帮助的。最重要的是能把生物移到易于
This is a special report by Prof. Tra- ger at the 6th International Conference on Protozoology held in Warsaw, Poland on July 5-11, 1981. This report covers the recent work on parasitic protozoa. Cultivation is not an end in itself, but a method of achieving other ends. Prior to P. falciparum culture, chickens infected and spleens only with human infection and from the Newcomer nocturnal monkey after 1965 were used as the source of this protozoan. Now that we have been able to pass protozoa through successive, continuous growth passages in human erythrocytes in vitro, it is possible to conduct a molecular and cellular biology study of Plasmodium falciparum in the laboratory. By culturing, more protozoa are generally available, which is of great help to some biochemical and immunological and serological studies. The most important thing is to be able to move the creature to easy