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目的了解解脲支原体(UU)和沙眼衣原体(CT)感染与不孕不育的关系,同时了解不孕不育夫妇之间的交叉感染情况,为生殖医学提供相关的医学依据,以便采取相应的治疗和有效预防措施,提高受孕率。方法用荧光定量PCR方法检测200对夫妇(不孕不育组100对,正常对照组100对)女性宫颈分泌物、男性精液标本中的解脲支原体一DNA和沙眼衣原体一DNA含量,并对相关实验数据进行医学统计学处理。结果不孕不育组女性解脲支原体-DNA阳性率46%,沙眼衣原体-DNA阳性率25%,解脲支原体或沙眼衣原体的总感染率为55%,男性解脲支原体-DNA阳性率21%,沙眼衣原体-DNA阳性率20%,解脲支原体或沙眼衣原体的总感染率为36%,一对夫妇同时感染同一种病原体者32%;正常对照组女性则分别为15%、9%、20%,男性分别为12%、6%、15%,一对夫妇同时感染同一种病原体者11%,两组间有显著性差异。结论解脲支原体和沙眼衣原体是引起男女不孕不育的重要病原体之一,应引起生殖医学工作者的足够重视,同时积极加强不孕不育夫妇之间交叉感染的预防,也是提高不孕不育夫妇受孕率的方法之一。
Objective To understand the relationship between UU and CT infection and infertility, and to understand the cross-infection among infertile couples and to provide relevant medical evidences for reproductive medicine in order to take the corresponding Treatment and effective preventive measures to improve the pregnancy rate. Methods The DNA content of urogenital mycoplasma-DNA and chlamydia trachomatis in 200 female infertility group (100 pairs of infertility group and 100 pairs of normal control group) was detected by real-time fluorescence quantitative PCR. Experimental data for medical statistics. Results In infertility women, the positive rate of Ureaplasma urealyticum DNA was 46%, the positive rate of Chlamydia trachomatis-DNA was 25%, the total infection rate of Ureaplasma urealyticum or Chlamydia trachomatis was 55%, the positive rate of Ureaplasma urealyticum-DNA was 21% , The positive rate of Chlamydia trachomatis-DNA was 20%, the total infection rate of Ureaplasma urealyticum or Chlamydia trachomatis was 36%, while one couple was infected with 32% of the same pathogens at the same time; the women in the normal control group were 15%, 9%, 20% %, Men were 12%, 6%, 15%, a couple infected with the same pathogen at the same time 11%, there were significant differences between the two groups. Conclusion Ureaplasma urealyticum and Chlamydia trachomatis are one of the most important causative agents of infertility in men and women, which should be paid more attention to by reproductive medical workers. At the same time, it is necessary to strengthen the prevention of infertility among infertile couples and to improve infertility One of the ways to nurture a couple’s pregnancy rate.