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目的:分析结直肠癌HLA-DR表达及癌组织中树突细胞(DC)的密度,探讨两者对结直肠癌患者临床分期及预后的影响。方法:47例结直肠癌石蜡标本,应用免疫组织化学SP法检测癌组织HLA-DR表达和S100阳性树突细胞的浸润密度,并与患者的临床分期和预后进行统计学分析。结果:结直肠癌HLA-DR表达率27.7%(13/47),表达率随临床分期增加而逐渐下降(P<0.05),但对预后无明显影响。S-100阳性DC丰富(≥10/HPF)的病例为44.7%(21/47),DC浸润密度在患者的临床分期和预后方面均有显著差异性(P<0.05)。HLA-DR表达与DC浸润程度密切相关(r=0.522;P<0.01)。结论:HLA-DR表达与DC浸润密度可反映了结直肠癌患者的抗肿瘤免疫状况,可能成为判断临床分期和预后的重要指标。
OBJECTIVE: To analyze the expression of HLA-DR in colorectal cancer and the dendritic cell (DC) density in colorectal cancer and to investigate the impact of both on the clinical stage and prognosis of colorectal cancer. Methods: 47 cases of paraffin-embedded specimens of colorectal cancer were studied. The expression of HLA-DR and the density of S100-positive dendritic cells were detected by SP immunohistochemistry. The clinical stage and prognosis were analyzed statistically. Results: The positive rate of HLA-DR in colorectal cancer was 27.7% (13/47). The expression rate of HLA-DR decreased with the increase of clinical stage (P <0.05), but had no significant effect on prognosis. There were 44.7% (21/47) patients with positive S-100 positive DC (≥10 / HPF), and the DC infiltration density was significantly different in both clinical stage and prognosis (P <0.05). HLA-DR expression was closely related to the degree of DC infiltration (r = 0.522; P <0.01). Conclusion: The expression of HLA-DR and DC infiltration density may reflect the antitumor immune status of patients with colorectal cancer, which may be an important index to judge the clinical stage and prognosis.