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目的克隆中国人肥胖基因，并进行序列分析和在大肠杆菌中表达。方法采用ＲＴＰＣＲ法从中国人脂肪组织ＲＮＡ中扩增出肥胖基因（Ｏｂｅｓｅｇｅｎｅ），进行核苷酸顺序分析，并将该基因克隆入原核表达载体，在大肠杆菌中表达。结果克隆了中国人肥胖基因，其ｃＤＮＡ顺序与已报道的白种人的序列完全一致，并成功在大肠杆菌中获得表达。结论本项工作为进一步研究肥胖基因在肥胖症和糖尿病等病人中的表达情况及探讨肥胖基因的作用机制打下了基础。
OBJECTIVE: To clone Chinese obese genes and to analyze the sequence and express in Escherichia coli. Methods Obese gene was amplified from Chinese adipose tissue RNA by RTPCR method. The nucleotide sequence was analyzed. The gene was cloned into prokaryotic expression vector and expressed in E. coli. Results The Chinese obese gene was cloned. The sequence of cDNA was exactly the same as that reported in Caucasian and successfully expressed in E. coli. Conclusion This work laid the foundation for the further study of the expression of obesity gene in obesity and diabetes patients and the mechanism of action of obesity gene.