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目的探讨朝藿定C对骨质疏松模型小鼠骨组织形态学的影响。方法以0.4%戊巴比妥钠麻醉动物后,造模小鼠切除双侧卵巢,对照小鼠切除卵巢旁同样大小的脂肪团。术后45d将小鼠分为6组:假手术组(等容积常水)、模型组(等容积常水)、阳性药组(己烯雌酚2mg/kg)、朝藿定C高剂量组(20mg/kg)、朝藿定C中剂量组(10mg/kg)、朝藿定C低剂量组(5mg/kg),每组14只小鼠。各组动物按各自药物和剂量灌胃给药,每日1次。连续给药60d后,处死小鼠,以BI-2000医学图像分析系统对股骨做骨组织形态计量学分析。结果与对照组比较,模型组骨小梁平均宽度及骨小梁面积百分率、成骨细胞数明显减少,破骨细胞数明显增多,差异显著;与模型组比较,朝藿定C高中剂量组及己烯雌酚组骨小梁平均宽度及骨小梁面积百分率、成骨细胞数明显增高,破骨细胞数明显减少(P<0.05或P<0.01)。结论朝藿定C能增加去卵巢小鼠骨的骨量,具有促进骨形成和抑制骨吸收的双重作用。
Objective To investigate the effects of epigallocate C on morphological changes of bone in osteoporosis model mice. Methods After the animals were anesthetized with 0.4% sodium pentobarbital, bilateral ovaries were resected in the model mice and the same size of cellulite was resected in the control mice. The mice were divided into 6 groups at the 45th day postoperatively: sham operation group (constant volume of normal water), model group (constant volume of normal water), positive drug group (diethylstilbestrol 2mg / kg), epimedin C high dose group kg), Epimedin C medium dose group (10mg / kg), Epimedin C low dose group (5mg / kg), each group of 14 mice. Each group of animals according to their respective drug and dose gavage, 1 day. After continuous administration for 60 days, the mice were sacrificed and bone histomorphometry analysis of the femur was performed with BI-2000 medical image analysis system. Results Compared with the control group, the mean trabecular area and trabecular area percentage, the number of osteoblasts, the number of osteoblasts and the number of osteoclasts in the model group were significantly increased. Compared with the model group, Diethylstilbestrol group trabecular average width and trabecular area percentage, the number of osteoblasts was significantly increased, the number of osteoclasts was significantly reduced (P <0.05 or P <0.01). Conclusion Epimedin C can increase the bone mass of ovariectomized mice and has the dual role of promoting bone formation and inhibiting bone resorption.