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目的探讨人胚胎发育后期小脑皮层内一氧化氮合酶(NOS)阳性神经元的生长、发育规律。方法采用免疫组织化学方法观察人胚胎发育后期小脑皮层组织内NOS阳性神经元的发育过程。结果第6~7个月龄时,小脑室管层内可见NOS阳性神经元细胞,核多呈圆形,细胞有短小突起。第8~9个月龄时,小脑中间层可见NOS阳性神经元细胞,核呈圆形、椭圆形或梭形,细胞体积增大,细胞质明显增多,染色加深,膨体纤维呈串珠状,伸向边缘层。第10个月龄时,边缘层变厚,主要由5~6层排列较紧密的NOS阳性神经元细胞构成,其中也有体积较小的NOS阳性神经元细胞,神经纤维变细。结论小脑内NOS阳性神经细胞产生的一氧化氮,对小脑内神经元和神经胶质细胞的分化、增殖、迁移等具有重要作用。
Objective To investigate the growth and development of nitric oxide synthase (NOS) positive neurons in cerebellar cortex of human embryonic development. Methods Immunohistochemistry was used to observe the development of NOS positive neurons in the cerebellar cortex of human embryos. Results At the 6th to 7th month of age, NOS positive neurons were found in the cerebellar ventricular layer. The nuclei were mostly round with short protrusions. At the 8th to 9th month of age, NOS positive neurons were observed in the middle cerebellar surface. The nuclei were round, oval or fusiform in shape. The cell volume increased, the cytoplasm increased obviously, the staining deepened. To the edge layer. At the age of 10 months, the marginal layer became thickened and mainly consisted of 5 to 6 closely arranged NOS positive neurons, including smaller NOS positive neurons and thinner nerve fibers. Conclusion Nitric oxide produced by NOS positive neurons in the cerebellum plays an important role in the differentiation, proliferation and migration of neurons and glial cells in cerebellum.