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MTT Cell Proliferation Assay was used to optimize the concentration of Telomerase Restrictors(TRs) with minimum tox-icity to the selected cells. FACSort flow cytometer and Innotest P24 HIV(Human immunodeficiency Virus) antigen mAb ELISA Kit were used to investigate the anti-HIV-1 activities of TRs. The results showed that TRs had low cytotoxicity to the PBMC (Pe-ripheral Blood mononuclear cells) and CEM/GFP if the concentra-tion of TRs was at 50 μmol/L or below, and the supernatant from PBMC pretreated with SHIV and TR1-001 /TR1-002 could not infect the PBMC, while can infect the C8166 with reduced infec-tivity, which suggested that the TRs may be one of the novel re-sources for screening anti-HIV-1 agents.
MTT Cell Proliferation Assay was used to optimize the concentration of Telomerase Restrictors (TRs) with minimum tox-icity to the selected cells. FACSort flow cytometer and Innotest P24 HIV (Human immunodeficiency Virus) antigen mAb ELISA Kit were used to investigate the anti-HIV -1 activities of TRs. The results showed that TRs had low cytotoxicity to the PBMC (Pe-ripheral Blood mononuclear cells) and CEM / GFP if the concentra tion of TRs was at 50 μmol / L or below, and the supernatant from PBMC pretreated with SHIV and TR1-001 / TR1-002 could not infect the PBMC, while can infect the C8166 with reduced infectivity, which suggested that the TRs may one of the novel re-sources for screening anti-HIV-1 agents .