研究在离体条件下各种单体柴胡皂甙和甘草甜素抑制Ｎａ＋，Ｋ＋ＡＴＰ酶活性的构效关系。实验结果表明，各种柴胡皂甙抑制Ｎａ＋，Ｋ＋ＡＴＰ酶活性的作用强度依次为：ｂ１＞ｄ＞ｂ２＞ｂ４＞ａ＞ｂ３＞ｅ＞ｃ。柴胡皂甙化学结构中的Ｃ２３ＯＨ，Ｃ１６ＯＨ及Ｃ１１和Ｃ１３的共轭双烯可能对其抑制活性起重要作用。甘草甜素（ＧＬ），甘草次酸（ＧＡ）和生胃酮（１８β甘草次酸半琥珀酸双钠盐，ＣＸ）抑制Ｎａ＋，Ｋ＋ＡＴＰ酶活性的作用强度依次为ＧＡ≥ＣＸ＞ＧＬ。研究还证明，柴胡皂甙ｄ对Ｎａ＋，Ｋ＋ＡＴＰ酶的抑制为非竟争性抑制 To study the structure-activity relationship of various saikosaponins and glycyrrhizin inhibiting Na +, K + -ATPase activity under ex vivo conditions. The experimental results showed that the inhibitory effects of various saikosaponins on the activities of Na + and K + -ATPase were as follows: b1> d> b2> b4> a> b3> e> c. The chemical structure of saikosaponin C23  OH, C16  OH and C11 and C13 conjugated dienes may play an important role in its inhibitory activity. The intensities of inhibition of Na + and K + -ATPase activity by glycyrrhizin (GL), glycyrrhetinic acid (GA) and carbenoxolone (18 β-glycyrrhetinic acid hemisuccinate bis-sodium salt, CX) were GA ≥ CX > GL. Studies also show that saikosaponin d Na +, K + ATP enzyme inhibition of non-competitive inhibition