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目的:研究局灶性脑缺血再灌注后细胞凋亡、HSP70蛋白表达时空规律以及外源VEGF及VEGF抗体对它们的影响,探讨VEGF对缺血再灌注损伤的保护作用及其机制。方法:采用原位末端标记(TUNEL)、免疫组化方法,研究局灶性脑缺血再灌注后细胞凋亡数及HSP70蛋白表达时空分布,采用脑表面使用VEGF及侧脑室注射VEGF抗体,观察内外源VEGF对它们的影响。结果:VEGF抗体能显著增加缺血侧脑组织凋亡细胞数(再灌注12h-7d)及HSP70表达量(再灌注1-3d),而外源VEGF因子能显著减少同侧脑组织凋亡细胞(再灌注全程)及HSP70表达量(再灌注1-3d)。结论:VEGF因子可抑制缺血脑组织细胞凋亡及HSP70表达量,提示VEGF参与保护缺血性脑损伤。
OBJECTIVE: To study the effects of VEGF on the apoptosis and the expression of heat shock protein 70 (HSP70) in focal cerebral ischemia / reperfusion and the protective effect of exogenous VEGF and VEGF antibodies on the protective effect of VEGF on ischemia-reperfusion injury and its mechanism. Methods: TUNEL and immunohistochemical methods were used to study the number of apoptotic cells and the spatial and temporal distribution of HSP70 protein after focal cerebral ischemia / reperfusion. VEGF and lateral ventricle were used to observe VEGF Effects of exogenous and exogenous VEGF on them. RESULTS: VEGF antibody significantly increased the number of apoptotic cells (12h-7d after reperfusion) and the expression of HSP70 (1-3d after reperfusion) in the ischemic brain tissue, while the exogenous VEGF could significantly reduce the number of apoptotic cells in ipsilateral brain (Whole reperfusion) and the expression of HSP70 (1-3d reperfusion). Conclusion: VEGF can inhibit the apoptosis of ischemic brain tissue and the expression of HSP70, suggesting that VEGF is involved in the protection of ischemic brain injury.