为探讨胶质源性神经营养因子（ＧＤＮＦ）在中枢神经系统中的表达，采用免疫组化法检测该因子在成年大鼠中枢神经系统部分区域的分布。结果发现大脑皮质各层有极弱阳性的神经元；小脑皮质分子层及蒲肯野细胞呈强阳性反应；脊髓灰质中阳性神经元广为分布，尤以前角、后角Ⅰ、Ⅱ板层及Ｃｌａｒｋ核明显。此外，脊髓胶质细胞和中央管室管膜上皮均有ＧＤＮＦ阳性信号表达，表明ＧＤＮＦ对成年大鼠中枢神经系统内的多种神经元群均有功能作用。 To investigate the expression of glial derived neurotrophic factor (GDNF) in the central nervous system, immunohistochemistry was used to detect the distribution of GDNF in a part of the central nervous system in adult rats. The results showed that there were very weakly positive neurons in the cerebral cortex. The cerebellar cortical laminae and Purkinje cells were strongly positive. The positive neurons in the spinal cord were widely distributed, especially in the anterior horn and posterior horn. Clark verified. In addition, GDNF positive signals were expressed in both glia and central tubular epithelial cells, indicating that GDNF has a functional role in various neuronal populations in the central nervous system of adult rats.